Relationship of F.IX specific activity to circulating F.IX levels in transgenic mice
Transgenic line . | Circulating F.IX levels in mice, ng/mL . | Activity, U/mL* . | Specific activity, U/mg† . |
|---|---|---|---|
| WT, less than 10 μg/mL | 4309 ± 1821 | 1.40 ± 0.58 | 341.00 ± 101.82 |
| WT, greater than 10 μg/mL | 17 238 ± 9759 | 3.77 ± 2.76 | 208.00 ± 51.84 |
Transgenic line . | Circulating F.IX levels in mice, ng/mL . | Activity, U/mL* . | Specific activity, U/mg† . |
|---|---|---|---|
| WT, less than 10 μg/mL | 4309 ± 1821 | 1.40 ± 0.58 | 341.00 ± 101.82 |
| WT, greater than 10 μg/mL | 17 238 ± 9759 | 3.77 ± 2.76 | 208.00 ± 51.84 |
hF.IX activity is determined in an aPTT assay on plasma from the transgenic mice, and hF.IX antigen levels are determined by ELISA.
As determined by one-stage aPTT assay.
Specific activity of hF.IX in murine (F.IX-deficient plasma, 470 ± 150 U/mg protein) determined as described in “Analysis of hF.IX protein.”