Analysis of hemophilia B knockout mice carrying wild-type and mutant hF.IX transgenes
Line . | Copy no. . | RT-PCR . | Northern blot, % of human liver RNA . | hF.IX levels, μg/mL . | aPTT, sec . |
|---|---|---|---|---|---|
| ES-2 | 68 | + | 13 | 0.0 | 55-65 |
| ES-13 | 12 | + | 10 | 0.0 | 55-65 |
| LS-37 | 6 | + | 408 | 0.0 | 55-65 |
| LS-64 | 7 | + | 129 | 0.0 | 55-65 |
| cCH-4 | 13 | + | 20 | 0.0 | 55-65 |
| cCH-6 | 10 | + | ND | 0.0 | 55-65 |
| MS-2 | 15 | + | 255 | 8.0-10.0 | 55-65 |
| MS-12 | 1 | + | ND | 0.5-1.6 | 55-65 |
| WT-2 | 2 | + | 110 | 3.0-5.0 | 36-38 |
| WT-7 | 14 | + | 713 | 6.0-12.0 | 36-38 |
| WT-14 | 3 | + | 388 | 20.0-40.0 | 36-38 |
Line . | Copy no. . | RT-PCR . | Northern blot, % of human liver RNA . | hF.IX levels, μg/mL . | aPTT, sec . |
|---|---|---|---|---|---|
| ES-2 | 68 | + | 13 | 0.0 | 55-65 |
| ES-13 | 12 | + | 10 | 0.0 | 55-65 |
| LS-37 | 6 | + | 408 | 0.0 | 55-65 |
| LS-64 | 7 | + | 129 | 0.0 | 55-65 |
| cCH-4 | 13 | + | 20 | 0.0 | 55-65 |
| cCH-6 | 10 | + | ND | 0.0 | 55-65 |
| MS-2 | 15 | + | 255 | 8.0-10.0 | 55-65 |
| MS-12 | 1 | + | ND | 0.5-1.6 | 55-65 |
| WT-2 | 2 | + | 110 | 3.0-5.0 | 36-38 |
| WT-7 | 14 | + | 713 | 6.0-12.0 | 36-38 |
| WT-14 | 3 | + | 388 | 20.0-40.0 | 36-38 |
ND indicates not determined by Northern blot.