HIV-1BaL exposure does not inhibit cytokine secretion by CD40L stimulation
. | Median production, pg/mL (range) . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | IL-12 p70 . | . | TNFα . | . | |||
. | DCs . | Control DCs* . | DCs . | Control DCs* . | |||
| Medium | 0.5 (0-1.7) | 0 | 0.5 (0-1.0) | 0.3 (0-0.6) | |||
| 10 × BaL | 0.3 (0-1.0) | 0 | 12.3† (2.2-527) | 20.2 (5.6-34.7) | |||
| 10 × BaL washed off | 0.2* (0-0.4) | 0.9 (0-1.8) | 8.7* (0-17.4) | 7.6 (4.9-10.3) | |||
| CD40L | 1336‡ (658-28 258) | 1076 (1021-1160) | 7441‡ (3313-36 329) | 5576 (3967-7186) | |||
| 10 × BaL CD40L | 2061‡ (458-17 139) | 2103 (2019-2187) | 10 387‡ (6386-107 607) | 11 313 (6376-16 250) | |||
| 10 × BaL washed off CD40L | 3699* (3612-3786) | 3821 (2431-5210) | 12 852* (9454-16 250) | 15 135 (6160-24 110) | |||
. | Median production, pg/mL (range) . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | IL-12 p70 . | . | TNFα . | . | |||
. | DCs . | Control DCs* . | DCs . | Control DCs* . | |||
| Medium | 0.5 (0-1.7) | 0 | 0.5 (0-1.0) | 0.3 (0-0.6) | |||
| 10 × BaL | 0.3 (0-1.0) | 0 | 12.3† (2.2-527) | 20.2 (5.6-34.7) | |||
| 10 × BaL washed off | 0.2* (0-0.4) | 0.9 (0-1.8) | 8.7* (0-17.4) | 7.6 (4.9-10.3) | |||
| CD40L | 1336‡ (658-28 258) | 1076 (1021-1160) | 7441‡ (3313-36 329) | 5576 (3967-7186) | |||
| 10 × BaL CD40L | 2061‡ (458-17 139) | 2103 (2019-2187) | 10 387‡ (6386-107 607) | 11 313 (6376-16 250) | |||
| 10 × BaL washed off CD40L | 3699* (3612-3786) | 3821 (2431-5210) | 12 852* (9454-16 250) | 15 135 (6160-24 110) | |||
Immature DCs or control DCs devoid of T cells were exposed to 10× HIV-1BaL for 72 hours or the virus was washed off after overnight incubation and then cultured for a total of 72 hours, with or without CD40L stimulation during the final 24 hours. Cell culture supernatants were collected and analysed simultaneously by Luminex for the presence of TNFα and IL-12 p70. n = 6, if not otherwise indicated.
Data obtained from 2 individual donors (n = 2). Statistical analysis was not performed due to limited number of samples.
A significant difference between medium and HIV-1BaL -exposed DCs (P < .01, Mann-Whitney).
A significant difference between CD40L-stimulated DCs and the matched unstimulated DCs (P < .01, Mann-Whitney).