Flow cytometric analysis on RCC-reactive CTL clones B5, G16, K34, and J23
. | B5 . | G16 . | K34 . | J23 . |
|---|---|---|---|---|
| CD3 | 95 | 99 | 97 | 100 |
| CD8 | 95 | 99 | 97 | 100 |
| CD4dim | 2 | 3 | 73 | 0 |
| TCRαβ | 91 | 99 | 96 | 100 |
| TCRγδ | 2 | 1 | 0 | 0 |
| CD16 | 1 | 0 | 0 | 0 |
| CD56dim | 17 | 44 | 46 | 44 |
| CD57 | 1 | 1 | NT | 1 |
| CD45RA | 4 | 1 | 0 | 1 |
| CD45RO | 68 | 99 | 82 | 62 |
| CCR7dim | 26 | 7 | 18 | 10 |
| CD28dim | 46 | 48 | NT | 52 |
| CD94 | 13 | 33 | 17 | 1 |
| CD158a | 3 | 5 | 5 | 2 |
| CD158b | 5 | 14 | 4 | 2 |
| CD25 | 67 | 85 | NT | 95 |
| CD69 | 71 | 67 | NT | 93 |
| HLA-DR | 90 | 92 | NT | 79 |
. | B5 . | G16 . | K34 . | J23 . |
|---|---|---|---|---|
| CD3 | 95 | 99 | 97 | 100 |
| CD8 | 95 | 99 | 97 | 100 |
| CD4dim | 2 | 3 | 73 | 0 |
| TCRαβ | 91 | 99 | 96 | 100 |
| TCRγδ | 2 | 1 | 0 | 0 |
| CD16 | 1 | 0 | 0 | 0 |
| CD56dim | 17 | 44 | 46 | 44 |
| CD57 | 1 | 1 | NT | 1 |
| CD45RA | 4 | 1 | 0 | 1 |
| CD45RO | 68 | 99 | 82 | 62 |
| CCR7dim | 26 | 7 | 18 | 10 |
| CD28dim | 46 | 48 | NT | 52 |
| CD94 | 13 | 33 | 17 | 1 |
| CD158a | 3 | 5 | 5 | 2 |
| CD158b | 5 | 14 | 4 | 2 |
| CD25 | 67 | 85 | NT | 95 |
| CD69 | 71 | 67 | NT | 93 |
| HLA-DR | 90 | 92 | NT | 79 |
CTL clones were isolated from allogeneic MLTC-1 on blood-derived CD8+ T lymphocytes of healthy donor 860 and the HLA class la—matched RCC line MZ1257-RCC. T cells were stained with FITC- or PE-conjugated monoclonal antibodies directed against indicated cell surface markers and were then analyzed. Results shown represent percentage of cells with positive FITC- or PE-staining. Low staining intensity is marked as “dim.” NT indicates not tested.