Colonies from week-5 LTCs initiated with 1 × 105 Notch1-IC-, Notch4-IC-, and MIG-transduced cord cells
. | MIG, no. . | Notch1-IC, no. . | Notch4-IC, no. . |
|---|---|---|---|
| Cord 1 | 250 | 450 | NA |
| Cord 2 | 200 | 820 | NA |
| Cord 3 | 1730 | 2930 | NA |
| Cord 4 | 535 | 660 | NA |
| Cord 5 | 863 | 1730 | NA |
| Mean ± SD for Notch1-IC | 716 ± 626 | 1318 ± 1025* | NA |
| Cord 6 | 114 | NA | 245 |
| Cord 7 | 430 | NA | 1140 |
| Cord 8 | 910 | NA | 1290 |
| Cord 9 | 620 | NA | 860 |
| Cord 4 | 535 | NA | 700 |
| Mean ± SD for Notch4-IC | 522 ± 290 | NA | 847 ± 408* |
. | MIG, no. . | Notch1-IC, no. . | Notch4-IC, no. . |
|---|---|---|---|
| Cord 1 | 250 | 450 | NA |
| Cord 2 | 200 | 820 | NA |
| Cord 3 | 1730 | 2930 | NA |
| Cord 4 | 535 | 660 | NA |
| Cord 5 | 863 | 1730 | NA |
| Mean ± SD for Notch1-IC | 716 ± 626 | 1318 ± 1025* | NA |
| Cord 6 | 114 | NA | 245 |
| Cord 7 | 430 | NA | 1140 |
| Cord 8 | 910 | NA | 1290 |
| Cord 9 | 620 | NA | 860 |
| Cord 4 | 535 | NA | 700 |
| Mean ± SD for Notch4-IC | 522 ± 290 | NA | 847 ± 408* |
Lineage-depleted cord cells were retrovirally transduced with MIG (n = 9), Notch1-IC (n = 5), and Notch4-IC (n = 5) and sorted for GFP. First, 1 × 105 GFP+ cord cells were placed into LTCs. Cultures were maintained at 37°C in humidified incubators and had weekly media changes with long-term culture media. After 5 weeks, cultures were trypsinized and cells were plated into methylcellulose in the presence of IL-3, IL-6, G-CSF, GM-CSF, Steel factor, and erythropoietin, and colonies were counted 20 days after plating.
NA indicates not applicable.
P ≤ .05.