Level of engraftment of human cells in sublethally irradiated NOD/SCID-β2mnull mice
. | . | . | . | . | FISH . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Engraftment† . | . | . | Clonal cells/total . | . | Normal cells/total . | . | ||||
| Wks after transplantation . | Clonal marker (% of cytogenetically abnormal cells*) . | Blood . | Bone marrow . | Spleen . | Bone marrow (%) . | Spleen (%) . | Bone marrow . | Spleen . | ||||
| 4 | del(5q)/trisomy8 (95) | 58.37 | 4.44 | 71.54 | NA | 24/272 (8.8)§ | NA | 248/272 | ||||
| 6 | del(5q)/trisomy8; (95‡) | 26.06 | 37.7 | 27.0 | 6/61 (9.8)§ | 3/44 (6.8)§ | 55/61 | 41/44 | ||||
| 5 | −Y (75) | 0.73 | 2.11 | 14.91 | 0 (0.0) | 0 (0.0) | 100 | 100 | ||||
| 17 | −Y (75) | 0.61 | 1.19 | 0.09 | 22/213 (10.3) | 0 | 191/213 | 4 | ||||
| 6 | del(7q) (90) | 7.77 | 0.71 | 6.1 | 1/2 (50) | 21/218 (9.6) | 1/2 | 197/218 | ||||
| 13 | del(5q) (68) | 1.74 | 0.71 | 34.19 | 1/5 (20) | 13/201 (6.4) | 4/5 | 188/201 | ||||
| Mean ± SEM | — | 15.9 ± 9.4 | 7.8 ± 6.0 | 25.6 ± 10.5 | — | — | — | — | ||||
. | . | . | . | . | FISH . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Engraftment† . | . | . | Clonal cells/total . | . | Normal cells/total . | . | ||||
| Wks after transplantation . | Clonal marker (% of cytogenetically abnormal cells*) . | Blood . | Bone marrow . | Spleen . | Bone marrow (%) . | Spleen (%) . | Bone marrow . | Spleen . | ||||
| 4 | del(5q)/trisomy8 (95) | 58.37 | 4.44 | 71.54 | NA | 24/272 (8.8)§ | NA | 248/272 | ||||
| 6 | del(5q)/trisomy8; (95‡) | 26.06 | 37.7 | 27.0 | 6/61 (9.8)§ | 3/44 (6.8)§ | 55/61 | 41/44 | ||||
| 5 | −Y (75) | 0.73 | 2.11 | 14.91 | 0 (0.0) | 0 (0.0) | 100 | 100 | ||||
| 17 | −Y (75) | 0.61 | 1.19 | 0.09 | 22/213 (10.3) | 0 | 191/213 | 4 | ||||
| 6 | del(7q) (90) | 7.77 | 0.71 | 6.1 | 1/2 (50) | 21/218 (9.6) | 1/2 | 197/218 | ||||
| 13 | del(5q) (68) | 1.74 | 0.71 | 34.19 | 1/5 (20) | 13/201 (6.4) | 4/5 | 188/201 | ||||
| Mean ± SEM | — | 15.9 ± 9.4 | 7.8 ± 6.0 | 25.6 ± 10.5 | — | — | — | — | ||||
Results determined by flow cytometry (% CD45+ human cells) and FISH analysis for individual mice that underwent transplantation and which are illustrated in the insert. NA indicates not available; —, not applicable.
Clonal abnormality examined by FISH.
Percent of CD45+ human cells (illustrated in Figure 1A).
FISH results from spleen cells were based on CD45+CD33+ sorted samples; in the other mice FISH results were based on whole bone marrow and spleen cells.
Only human cells containing an isolated del(5q) were detectable; no cells containing trisomy 8 were identified (illustrated in Figure 1B).