Primers and probes used to quantify leukemic DA1-3b cells (M-BCR and albumin) and to evaluate B7-H1 and B7.1 expression
Name . | Sequence . |
|---|---|
| M-BCR | |
| Forward | 5′TCCGCTGACCATCAATAAGGA3 |
| Reverse | 5′CACTCAGACCCTGAGGCTCAA3 |
| Probe | 5′FAM-CCCTTCAGCGGCCAGTAGCATCTGA-TAMRA3′ |
| Albumin | |
| Forward | 5′TGTAGACCAGGTTGGCCTCG3 |
| Reverse | 5′AAGTAAAATCAGAAGCCAGGCGT3′ |
| Probe | 5′FAM-CGCACGCCTTTAGTCCCAGCACTG-TAMRA3′ |
| B7.1 | |
| Forward | 5′GCTGCTGATTCGTCTTTCACAA3′ |
| Reverse | 5′GGGCCACACTTTTAGTTTCCC3′ |
| Probe | 5′FAM TGCTGTCTGTCATTGC-MGB3′ |
| B7-H1 | |
| Forward | 5′GAGAGCCTCGCTGCCAAA3′ |
| Reverse | 5′AGTCCGCACCACCGTAGCT3′ |
| Probe | 5′FAM ACTGCTGCATAATCA-MGB3′ |
Name . | Sequence . |
|---|---|
| M-BCR | |
| Forward | 5′TCCGCTGACCATCAATAAGGA3 |
| Reverse | 5′CACTCAGACCCTGAGGCTCAA3 |
| Probe | 5′FAM-CCCTTCAGCGGCCAGTAGCATCTGA-TAMRA3′ |
| Albumin | |
| Forward | 5′TGTAGACCAGGTTGGCCTCG3 |
| Reverse | 5′AAGTAAAATCAGAAGCCAGGCGT3′ |
| Probe | 5′FAM-CGCACGCCTTTAGTCCCAGCACTG-TAMRA3′ |
| B7.1 | |
| Forward | 5′GCTGCTGATTCGTCTTTCACAA3′ |
| Reverse | 5′GGGCCACACTTTTAGTTTCCC3′ |
| Probe | 5′FAM TGCTGTCTGTCATTGC-MGB3′ |
| B7-H1 | |
| Forward | 5′GAGAGCCTCGCTGCCAAA3′ |
| Reverse | 5′AGTCCGCACCACCGTAGCT3′ |
| Probe | 5′FAM ACTGCTGCATAATCA-MGB3′ |
M-BCR indicates major breakpoint cluster.