Distribution of NK cells expressing inhibitory receptors for HLA-C and HLA-E
Subject . | CD 158a/b+CD94−* . | CD 158a/b+CD94+* . | CD 158a/b−CD94−* . | CD 158a/b−CD94+* . |
|---|---|---|---|---|
| 1 | 14.28 | 13.22 | 28.77 | 43.72 |
| 2 | 26.62 | 7.91 | 33.08 | 32.38 |
| 3 | 25.52 | 16.42 | 23.94 | 34.12 |
| 4 | 12.26 | 14.85 | 33.16 | 39.73 |
| 5 | 18.73 | 7.52 | 36.07 | 37.68 |
| 6 | 19.34 | 4.92 | 29.65 | 46.08 |
| 7 | 23.74 | 11.54 | 27.81 | 36.91 |
| 8 | 14.99 | 6.16 | 34.95 | 43.89 |
| Mean ± SD | 19.44 ± 5.06 | 10.31 ± 3.99 | 30.93 ± 3.83 | 39.31 ± 4.61 |
Subject . | CD 158a/b+CD94−* . | CD 158a/b+CD94+* . | CD 158a/b−CD94−* . | CD 158a/b−CD94+* . |
|---|---|---|---|---|
| 1 | 14.28 | 13.22 | 28.77 | 43.72 |
| 2 | 26.62 | 7.91 | 33.08 | 32.38 |
| 3 | 25.52 | 16.42 | 23.94 | 34.12 |
| 4 | 12.26 | 14.85 | 33.16 | 39.73 |
| 5 | 18.73 | 7.52 | 36.07 | 37.68 |
| 6 | 19.34 | 4.92 | 29.65 | 46.08 |
| 7 | 23.74 | 11.54 | 27.81 | 36.91 |
| 8 | 14.99 | 6.16 | 34.95 | 43.89 |
| Mean ± SD | 19.44 ± 5.06 | 10.31 ± 3.99 | 30.93 ± 3.83 | 39.31 ± 4.61 |
PBMCs were isolated from 8 healthy HIV-uninfected subjects and were stained with a PE cyanin 5.1-conjugated antibody directed to the NK cell marker CD56, PE-conjugated anti-CD 158a and -CD 158b antibodies, and FITC-conjugated anti-CD94 antibodies.
Mean ± SD values represent those of the subpopulations of NK cells from all subjects tested.
Percentages of 104 CD56+ cells expressing CD94, CD 158a, and CD 158b molecules were determined.