K-Cl cotransport activity in Sod2+/+, Sod2-/-, and antioxidant-treated Sod2-/- red cells
. | Sod2+/+ . | Sod2-/- . | P . | Sod2-/- Euk . | P . |
|---|---|---|---|---|---|
| MCV, fL | 52.5 ± 3.34 | 45.1 ± 0.53 | < .05 | 46.2 ± 0.60 | NS |
| Reticulocytes, % | 2.9 ± 0.6 | 18.2 ± 1.5 | < .01 | 11.0 ± 0.4 | < .01 |
| Na+, mmol/kg Hb | 25.5 ± 2.6 | 51.6 ± 3.1 | < .01 | 40.0 ± 3.4 | < .05 |
| K+, mmol/kg Hb | 740 ± 28 | 1285 ± 141 | < .05 | 1265 ± 184 | < .05 |
| Mg2+, mmol/kg Hb | 14.1 ± 1.7 | 42.1 ± 8.4 | < .05 | 29.8 ± 6.1 | NS |
| K-Cl cotransport, mmol/1013 cell × h | 8.03 ± 0.6 | 19.7 ± 3 | < .05 | 14.4 ± 3.4 | NS |
. | Sod2+/+ . | Sod2-/- . | P . | Sod2-/- Euk . | P . |
|---|---|---|---|---|---|
| MCV, fL | 52.5 ± 3.34 | 45.1 ± 0.53 | < .05 | 46.2 ± 0.60 | NS |
| Reticulocytes, % | 2.9 ± 0.6 | 18.2 ± 1.5 | < .01 | 11.0 ± 0.4 | < .01 |
| Na+, mmol/kg Hb | 25.5 ± 2.6 | 51.6 ± 3.1 | < .01 | 40.0 ± 3.4 | < .05 |
| K+, mmol/kg Hb | 740 ± 28 | 1285 ± 141 | < .05 | 1265 ± 184 | < .05 |
| Mg2+, mmol/kg Hb | 14.1 ± 1.7 | 42.1 ± 8.4 | < .05 | 29.8 ± 6.1 | NS |
| K-Cl cotransport, mmol/1013 cell × h | 8.03 ± 0.6 | 19.7 ± 3 | < .05 | 14.4 ± 3.4 | NS |
Data presented are mean ± standard error. ANOVA with Dunnett multiple comparison test was used to compare Sod2+/+ with Sod2-/- and Sod2-/- treated with Euk-189 for 28 days. For ion measurements: Sod2+/+, n = 5; Sod2-/-, n = 7; Sod2-/- Euk-189, n = 5; except for Mg2+: Sod2+/+, n = 4; and Sod2-/-, n = 6. For MCV and reticulocyte count, n = 4 for all groups. Red cell ion content was measured in red cell hemolysate using atomic absorption spectrophotometry. The activity of the K-Cl cotransport was measured by Cl-dependent K efflux in intact red blood cells as described.36
NS indicates not significant.