Primers and TaqMan probes used for folate polymorphisms
SNP (RS no.), probe/primer . | 5′-3′ sequence . |
|---|---|
| TYMS 3R → 2R | |
| F | GTGGCTCCTGCGTTTCCCCC |
| R | CCGCGCCATGCCTGTGGCCGGCTCGGAGC |
| TYMS 1494del6 (16430) | |
| F | AGCTGAGTAACACCATCGATCATG |
| R | GGACGAATGCAGAACACTTCTTTA |
| AAGTTA | VIC-TGGTTATGAACTTTAAAGTT |
| Deletion | 6FAM-TGGTTATGAACTTTATAGTTG |
| TYMS IVS6 -68 C>T (1059394) | |
| F | TGCCATAATTGTACGACCTGTTGT |
| R | AGAACTTTGTTGATCACATCCTGTGT |
| C | VIC-CTCATGTCCGTGAAAC |
| T | 6FAM-CTCATGTCCATGAAAC |
| TYMS 1053C>T (699517) | |
| F | GAAATGGCTGTTTAGGGTGCTTT |
| R | CCCAACCCCTAAAGACTGACAATA |
| C | VIC-AAGGAGCTCGAAGGA |
| T | 6FAM-CAAAGGAGCTTGAAGG |
| TYMS 1122A>G (2790) | |
| F | GGATGCCGAGGTAAAAGTTCTTT |
| R | GATAGGTCACGGACAGATTTTTGA |
| A | VIC-CCTAGTTCCTTTTTCTTT |
| G | 6FAM-CCTAGTTCCTTCTTCTT |
| MTHFR 677C>T (1801133) | |
| F | CGGTGCATGCCTTCACAA |
| R | CTGACCTGAAGCACTTGAAGGA |
| C | VIC-TGATGAAATCGGCTCC |
| T | 6FAM-ATGATGAAATCGACTCC |
| MTHFR 1298A>C (1801131) | |
| F | CCCGAGAGGTAAAGAACAAAGACTT |
| R | GGAGGAGCTGCTGAAGATGTG |
| A | VIC-CAAAGACACTTTCTTC |
| C | 6FAM-AGACACTTGCTTCACT |
| SHMTI 1420C>T (1979277) | |
| F | CAGAGCCACCCTGAAAGAGTTC |
| R | AGTGGGCCCGCTCCTTTA |
| T | VIC-CGCCTCTTTCTTC |
| C | 6FAM-CGCCTCTCTCTTC |
| MTR 2756A>G (1805087) | |
| F | GAATACTTTGAGGAAATCATGGAAGA |
| R | TCTGTTTCTACCACTTACCTTGAGAGACT |
| A | VIC-AGACAGGACCATTATG |
| G | 6FAM-ACAGGGCCATTATG |
| RFC 80G>A (1051266) | |
| F | GGCCTGACCCCGAGCT |
| R | AGCCGTAGAAGCAAAGGTAGCA |
| G | VIC-CACGAGGCGCCGC |
| A | 6FAM-CGAGGTGCCGCCAG |
SNP (RS no.), probe/primer . | 5′-3′ sequence . |
|---|---|
| TYMS 3R → 2R | |
| F | GTGGCTCCTGCGTTTCCCCC |
| R | CCGCGCCATGCCTGTGGCCGGCTCGGAGC |
| TYMS 1494del6 (16430) | |
| F | AGCTGAGTAACACCATCGATCATG |
| R | GGACGAATGCAGAACACTTCTTTA |
| AAGTTA | VIC-TGGTTATGAACTTTAAAGTT |
| Deletion | 6FAM-TGGTTATGAACTTTATAGTTG |
| TYMS IVS6 -68 C>T (1059394) | |
| F | TGCCATAATTGTACGACCTGTTGT |
| R | AGAACTTTGTTGATCACATCCTGTGT |
| C | VIC-CTCATGTCCGTGAAAC |
| T | 6FAM-CTCATGTCCATGAAAC |
| TYMS 1053C>T (699517) | |
| F | GAAATGGCTGTTTAGGGTGCTTT |
| R | CCCAACCCCTAAAGACTGACAATA |
| C | VIC-AAGGAGCTCGAAGGA |
| T | 6FAM-CAAAGGAGCTTGAAGG |
| TYMS 1122A>G (2790) | |
| F | GGATGCCGAGGTAAAAGTTCTTT |
| R | GATAGGTCACGGACAGATTTTTGA |
| A | VIC-CCTAGTTCCTTTTTCTTT |
| G | 6FAM-CCTAGTTCCTTCTTCTT |
| MTHFR 677C>T (1801133) | |
| F | CGGTGCATGCCTTCACAA |
| R | CTGACCTGAAGCACTTGAAGGA |
| C | VIC-TGATGAAATCGGCTCC |
| T | 6FAM-ATGATGAAATCGACTCC |
| MTHFR 1298A>C (1801131) | |
| F | CCCGAGAGGTAAAGAACAAAGACTT |
| R | GGAGGAGCTGCTGAAGATGTG |
| A | VIC-CAAAGACACTTTCTTC |
| C | 6FAM-AGACACTTGCTTCACT |
| SHMTI 1420C>T (1979277) | |
| F | CAGAGCCACCCTGAAAGAGTTC |
| R | AGTGGGCCCGCTCCTTTA |
| T | VIC-CGCCTCTTTCTTC |
| C | 6FAM-CGCCTCTCTCTTC |
| MTR 2756A>G (1805087) | |
| F | GAATACTTTGAGGAAATCATGGAAGA |
| R | TCTGTTTCTACCACTTACCTTGAGAGACT |
| A | VIC-AGACAGGACCATTATG |
| G | 6FAM-ACAGGGCCATTATG |
| RFC 80G>A (1051266) | |
| F | GGCCTGACCCCGAGCT |
| R | AGCCGTAGAAGCAAAGGTAGCA |
| G | VIC-CACGAGGCGCCGC |
| A | 6FAM-CGAGGTGCCGCCAG |
RS indicates reference SNP.