Table 1.

Development of endothelial colonies from cord blood cell subsets



No. of endothelial colonies
Subset
Exp no. 1
Exp no. 2
Exp no. 3
Exp no. 4
CD34+  6   7   8   9  
CD34+CD11b+  5   12   4   7  
CD34+CD11b-
 
0
 
0
 
0
 
0*
 


No. of endothelial colonies
Subset
Exp no. 1
Exp no. 2
Exp no. 3
Exp no. 4
CD34+  6   7   8   9  
CD34+CD11b+  5   12   4   7  
CD34+CD11b-
 
0
 
0
 
0
 
0*
 

Number of endothelial colonies developing at 7 days of cultures from 1 × 105 CD34+, CD34+CD11b+, and CD34+CD11b- cord blood subsets, purified by cell sorting. Each experiment represents the analysis of cell subsets purified from individual cord blood donors. All cultures were monitored for up to 6 weeks. Scoring for the presence of endothelial colonies was performed in double blind by 2 independent investigators at 7 days, a culture period at which individual colonies could still be identified before becoming confluent.

Exp indicates experiment.

*

One experiment in which CD34+CD11b- cells developed 1 small endothelial colony after 3 weeks of culture.

Close Modal

or Create an Account

Close Modal
Close Modal