Higher ratios of full-length genomic to truncated subgenomic viral RNA correlate with higher globin vector titers
Transfer vector . | HeLa titer, TU/mL, mean ± SEM . | Full-length genomic RNA . | Truncated subgenomic RNA . |
|---|---|---|---|
| MSCV-GFP | 4.4 ± 0.7 × 107 | +++ | ± |
| d432βΔγm | 1.6 ± 0.5 × 107 | +++++ | ± |
| mLARβΔγV1 | 3.8 ± 1.8 × 105 | + | ++++ |
| mLARβΔγV3 | 5.5 × 105 | ++ | +++ |
| mLARβΔγV4 | 7.6 ± 5.5 × 105 | +++ | + |
| mLARβΔγV5 | 1.8 ± 0.5 × 106 | +++ | + |
Transfer vector . | HeLa titer, TU/mL, mean ± SEM . | Full-length genomic RNA . | Truncated subgenomic RNA . |
|---|---|---|---|
| MSCV-GFP | 4.4 ± 0.7 × 107 | +++ | ± |
| d432βΔγm | 1.6 ± 0.5 × 107 | +++++ | ± |
| mLARβΔγV1 | 3.8 ± 1.8 × 105 | + | ++++ |
| mLARβΔγV3 | 5.5 × 105 | ++ | +++ |
| mLARβΔγV4 | 7.6 ± 5.5 × 105 | +++ | + |
| mLARβΔγV5 | 1.8 ± 0.5 × 106 | +++ | + |
Unconcentrated globin vector titers were determined by genome transfer to HeLa cells as described in “Materials and methods.” The relative amounts of the indicated RNA species were visually estimated with levels ranging from minimal (±) to high (+++++).