Polarization and migration of CD34+ cells depend on PI3K activity
. | % polarized cells . | n . | % living cells . | n . | % migrated cells . | n . |
|---|---|---|---|---|---|---|
| Culture condition d0-d1; values d1 | ||||||
| Serum-free + GF | 64.90 ± 18.70 | 7 | 97.37 ± 1.84 | 3 | NE | 0 |
| Serum-free | 37.75 ± 19.91 | 7 | 90.91 ± 1.91 | 3 | NE | 0 |
| Serum-free + GF + Ly294002 | 3.91 ± 1.47 | 3 | 92.55 ± 1.11 | 3 | NE | 0 |
| Serum + GF | 82.86 ± 5.84 | 7 | 96.74 ± 2.02 | 3 | NE | 0 |
| Serum | 42.84 ± 25.61 | 3 | 83.53 ± 16.22 | 3 | NE | 0 |
| Serum + GF + Ly294002 | 9.73 ± 3.20 | 3 | 91.05 ± 1.41 | 3 | NE | 0 |
| Culture condition d2-d3; values d3 | ||||||
| Serum + GF + SDF-1α | 73.10 ± 3.85 | 3 | 94.48 ± 1.51 | 3 | 15.6 ± 4.20 | 3 |
| Serum + GF + SDF-1α + Ly294002 | 14.60 ± 7.12 | 3 | 87.39 ± 4.74 | 3 | 3.3 ± 0.25 | 3 |
. | % polarized cells . | n . | % living cells . | n . | % migrated cells . | n . |
|---|---|---|---|---|---|---|
| Culture condition d0-d1; values d1 | ||||||
| Serum-free + GF | 64.90 ± 18.70 | 7 | 97.37 ± 1.84 | 3 | NE | 0 |
| Serum-free | 37.75 ± 19.91 | 7 | 90.91 ± 1.91 | 3 | NE | 0 |
| Serum-free + GF + Ly294002 | 3.91 ± 1.47 | 3 | 92.55 ± 1.11 | 3 | NE | 0 |
| Serum + GF | 82.86 ± 5.84 | 7 | 96.74 ± 2.02 | 3 | NE | 0 |
| Serum | 42.84 ± 25.61 | 3 | 83.53 ± 16.22 | 3 | NE | 0 |
| Serum + GF + Ly294002 | 9.73 ± 3.20 | 3 | 91.05 ± 1.41 | 3 | NE | 0 |
| Culture condition d2-d3; values d3 | ||||||
| Serum + GF + SDF-1α | 73.10 ± 3.85 | 3 | 94.48 ± 1.51 | 3 | 15.6 ± 4.20 | 3 |
| Serum + GF + SDF-1α + Ly294002 | 14.60 ± 7.12 | 3 | 87.39 ± 4.74 | 3 | 3.3 ± 0.25 | 3 |
Data are for CB-derived CD34+ cells; mean ± SD.
n indicates number of independent experiments; d0, freshly isolated cells; GF, growth factors (SCF, TPO, FLT3L); and NE, not estimated.