Primer sequences used for real-time PCR analysis of iron transporter transcripts
. | Forward primer (5′ to 3′) . | Reverse primer (5′ to 3′) . |
|---|---|---|
| HPRT | TTGTAGCCCTCTGTGTGCTCAAG | GCCTGACCAAGGAAAGCAAAGTC |
| DMT1 | ||
| (non-IRE) | GGACCTAGGGCATGTGGCAT | ACACAAGTGAGTCAGCGTGG |
| DMT1 | ||
| (+ IRE) | AGTGGTTTATGTCCGGGACC | TTTAACGTAGCCACGGGTGG |
| IREG1 | CGTCATTGCTGCTAGAATCG | AGACTGAAATCAATACGAGC |
. | Forward primer (5′ to 3′) . | Reverse primer (5′ to 3′) . |
|---|---|---|
| HPRT | TTGTAGCCCTCTGTGTGCTCAAG | GCCTGACCAAGGAAAGCAAAGTC |
| DMT1 | ||
| (non-IRE) | GGACCTAGGGCATGTGGCAT | ACACAAGTGAGTCAGCGTGG |
| DMT1 | ||
| (+ IRE) | AGTGGTTTATGTCCGGGACC | TTTAACGTAGCCACGGGTGG |
| IREG1 | CGTCATTGCTGCTAGAATCG | AGACTGAAATCAATACGAGC |