Table 4. Clonotypic B cells in WM are... | American Society of Hematology

Table 4.

Clonotypic B cells in WM are characterized as CD20⁺CD138⁻IgM⁺IgD⁺ and CD20⁺CD138⁺ cells

				Clonotypic B cells with different clonal isotype expression, %
Phenotypic marker and patient	Source	No. cells analyzed	Clonal frequencies, %	μ⁺δ⁺	μ⁺δ⁻
CD20⁺CD138⁻
WM1-06.1^*	BM	48	96	NA	NA
WM1-06.2^*	BM	96	58	NA	NA
WM1-09	BM	96	61	30	70
WM1-14	BM	96	39	NA	NA
WM1-11	BM	96	57	NA	NA
WM1-11	Blood	144	2	NA	NA
WM1-13	BM	72	65	35	65
WM1-13	Blood	72	69	39	61
IgM⁺IgD⁺
WM1-08	BM	72	77	64	36
WM1-11	BM	72	86	NA	NA
WM1-13	BM	72	89	31	69
WM1-13	Blood	72	81	42	58
CD20⁺CD138⁺
WM1-09	BM	48	92	4	96

				Clonotypic B cells with different clonal isotype expression, %
Phenotypic marker and patient	Source	No. cells analyzed	Clonal frequencies, %	μ⁺δ⁺	μ⁺δ⁻
CD20⁺CD138⁻
WM1-06.1^*	BM	48	96	NA	NA
WM1-06.2^*	BM	96	58	NA	NA
WM1-09	BM	96	61	30	70
WM1-14	BM	96	39	NA	NA
WM1-11	BM	96	57	NA	NA
WM1-11	Blood	144	2	NA	NA
WM1-13	BM	72	65	35	65
WM1-13	Blood	72	69	39	61
IgM⁺IgD⁺
WM1-08	BM	72	77	64	36
WM1-11	BM	72	86	NA	NA
WM1-13	BM	72	89	31	69
WM1-13	Blood	72	81	42	58
CD20⁺CD138⁺
WM1-09	BM	48	92	4	96

One hundred percent of clonotypic B cells expressed clonal μ transcript. Clonal frequency was calculated as the percentage of sorted cells that expressed clonal μ transcript over the total number of cells that showed positive PCR results to β2-microglobulin or universal immunoglobulin control.

NA indicates not applicable.

BM samples from WM1-06 were collected 9 months apart.

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