Combinations of primer sets for heminested PCR
. | PCR primers, sense/antisense . | . | |
|---|---|---|---|
| Transcript type . | First-stage PCR . | Second-stage PCR . | |
| μ, clonotypic | FR1c/Cμ | CDR2/CDR3 or CDR3R/Cμ | |
| μ, secreted, clonotypic | FR1c/CμS.1 | CDR3R/CμS | |
| μ, membrane bound, clonotypic | FR1c/CμM.1 | CDR3R/CμM | |
| σ, clonotypic | FR1c/Cσ | CDR3R/Cσ | |
| β2m, control | β2m 5′/β2m 3′ | β2m 5′int/β2m 3′ | |
| Universal immunoglobulin, control | FR1c/JHc | FR2/JHc | |
. | PCR primers, sense/antisense . | . | |
|---|---|---|---|
| Transcript type . | First-stage PCR . | Second-stage PCR . | |
| μ, clonotypic | FR1c/Cμ | CDR2/CDR3 or CDR3R/Cμ | |
| μ, secreted, clonotypic | FR1c/CμS.1 | CDR3R/CμS | |
| μ, membrane bound, clonotypic | FR1c/CμM.1 | CDR3R/CμM | |
| σ, clonotypic | FR1c/Cσ | CDR3R/Cσ | |
| β2m, control | β2m 5′/β2m 3′ | β2m 5′int/β2m 3′ | |
| Universal immunoglobulin, control | FR1c/JHc | FR2/JHc | |