Summary of primer sequences
Primer . | Sequence . |
|---|---|
| VH1 leader | 5′CCATGGACTGGACCTGGAG (G/C/A) A3′ |
| VH2 leader | 5′ATGGACA (T/C) ACTTTG (T/C) TCCAC3′ |
| VH3 leader | 5′CCATGGA (G/A) CT (T/G) GGGCTC3′ |
| VH4 leader | 5′ATGAA (A/G) CACCTGTGGTTCTTC3′ |
| VH5 leader | 5′GGTCAACCGCCATCCT3′ |
| VH6 leader | 5′ATGTCTGTCTCCTTCCTCATCTTC3′ |
| Cμ | 5′GGAATTCTCACAGGAGAC3′ |
| Cμs | 5′AGCTGTGTCGGACATGACC3′ |
| Cμm | 5′CACAGGTTCTCAAAGCCCTC3′ |
| Cδ | 5′GTGTCTGCACCCTGATAT3′ |
| Cγ | 5′GGGGAAGACCGATGGGCCCT3′ |
| Cα | 5′GAGGCTCAGCGGGAAGACCTT3′ |
| FR1c | 5′GGTGCAGCTG (G/C) (A/T) G (G/C) AGTC (G/A/T) GG3′ |
| FR2 | 5′TATGAATTCGGAAAGGGCCTGGAGTGG3′ |
| FR3 | 5′CCGAGGACACGGC (T/C) (C/G) TGTATTACTG3′ |
| JHc | 5′ACCTGAGGAGACGGTGACC (A/G) (G/T) (G/T) GT3′ |
| β2m 5′ | 5′CCAGCAGAGAATGGAAAGTC3′ |
| β2m 5′ int | 5′TGTCTTTCAGCAAGGACTGG3′ |
| β2m 3′ | 5′GATGCTGCTTACATGTCTCG3′ |
Primer . | Sequence . |
|---|---|
| VH1 leader | 5′CCATGGACTGGACCTGGAG (G/C/A) A3′ |
| VH2 leader | 5′ATGGACA (T/C) ACTTTG (T/C) TCCAC3′ |
| VH3 leader | 5′CCATGGA (G/A) CT (T/G) GGGCTC3′ |
| VH4 leader | 5′ATGAA (A/G) CACCTGTGGTTCTTC3′ |
| VH5 leader | 5′GGTCAACCGCCATCCT3′ |
| VH6 leader | 5′ATGTCTGTCTCCTTCCTCATCTTC3′ |
| Cμ | 5′GGAATTCTCACAGGAGAC3′ |
| Cμs | 5′AGCTGTGTCGGACATGACC3′ |
| Cμm | 5′CACAGGTTCTCAAAGCCCTC3′ |
| Cδ | 5′GTGTCTGCACCCTGATAT3′ |
| Cγ | 5′GGGGAAGACCGATGGGCCCT3′ |
| Cα | 5′GAGGCTCAGCGGGAAGACCTT3′ |
| FR1c | 5′GGTGCAGCTG (G/C) (A/T) G (G/C) AGTC (G/A/T) GG3′ |
| FR2 | 5′TATGAATTCGGAAAGGGCCTGGAGTGG3′ |
| FR3 | 5′CCGAGGACACGGC (T/C) (C/G) TGTATTACTG3′ |
| JHc | 5′ACCTGAGGAGACGGTGACC (A/G) (G/T) (G/T) GT3′ |
| β2m 5′ | 5′CCAGCAGAGAATGGAAAGTC3′ |
| β2m 5′ int | 5′TGTCTTTCAGCAAGGACTGG3′ |
| β2m 3′ | 5′GATGCTGCTTACATGTCTCG3′ |