For the APC sequences, mutations to Ala are indicated in boldface underlined text.

Derived from the concentrations of APC required for half-maximal inhibition of the staurosporine-induced apoptosis (Figure 2A).

Based on the APTT dose-response data determined for rwt APC and APC variants (0.5-32 nM) (Figure 1B).

Derived from the ratio of relative activities for cytoprotective and anticoagulant activities given in the previous 2 columns.

Based on apparent second-order rate constants determined previously. ^17,22 

Based on the amidolytic activity determined for rwt APC and APC variants (0.5-32 nM) (Figure 1A).

Based on the catalytic efficiency derived from Figure 4 for cleavage of the PAR-1 peptide (TR33-62) by rwt APC and APC variants (500 nM).

Recombinant wild-type APC (rwt APC) activity was defined as 100%, and values for mutant APC are given as percentage of rwt APC activity.

No detectable activity under the conditions of the assay.

Anticoagulant activity of S360A-APC is not due to proteolysis of factor Va and, in contrast to rwt APC, is independent of the incubation time of APC with the plasma. ²²