Table 2.

Proteins that are significantly modulated after ATRA treatment at 24, 72, and 96 hours through AACT analysis

Peptides sequenced
Protein expression†
Accession no.
Protein name
Functional classification
Mr, kDa*
pl*
24 h
72 h
96 h
mRNA expression‡
P04720   eEF1A-1   Protein synthesis   50.1   9.10   5   0.67   0.32   0.36   Unchanged∥24  
P29692   EF-1-δ   Protein synthesis   31.1   4.90   2   0.80   0.53   ND   Unchanged24  
AAH09865  eEF1γ   Protein synthesis   50.1   6.25   4   0.35   0.41   0.38   Unchanged∥ 
P46199   IF-2   Protein synthesis   81.3   6.71   2   ND   0.47   0.35   Unchanged24  
P04765   eIF4A-1   Protein synthesis   46.1   5.32   3   0.53   0.34§  0.40§  Unchanged∥ 
P55010   eIF5   Protein synthesis   49.2   5.41   2   ND   0.48   ND   Unchanged24  
P42655   14-3-3ϵ   Signal transduction   29.1   4.63   4   0.66   0.44   0.41§  Unknown  
P29312   14-3-3ζ/δ   Signal transduction   27.7   4.73   2   0.81   0.58   0.59   Unknown  
P01112   c-H-ras   Signal transduction   21.2   5.16   2   ND   ND   0.19   Unknown  
P00540   c-mos   Signal transduction   37.8   9.15   3   ND   0.43   0.38   Unknown  
P30153   PP2A   Signal transduction   65.0   4.96   2   0.70   0.35   0.41   Unknown  
Q13233   MEKK 1   Signal transduction   164   8.52   3   0.92   ND   0.64   Repressed24  
P23528   Cofilin, P18   Structural   18.5   8.22   9   0.51   0.64   0.52   Repressed24  
P32119   Peroxiredoxin 2   Cellular defense   21.9   5.66   5   ND   0.47   0.50   Unknown  
C28894   MPO   Cellular defense   92.4   9.25   9   0.35   0.52   0.48   Repressed23  
P25763   CDC42 homolog   Not defined   21.3   6.16   3   0.35   0.54   ND   Repressed24  
P10809   60-kDa hsp   Chaperone   61.0   5.70   7   0.77   0.67   0.48   Repressed23  
Q9NR31   SAR1a   Transport   22.4   6.22   4   ND   0.18   0.20   Unknown  
P12270   Nucleoprotein TPR   mRNA transport   266   5.01   6   0.88   0.50   0.48   Unknown  
P07910   hnRNP C1/C2   mRNA splicing   33.7   4.95   3   0.64   0.55   ND   Unchanged∥ 
Q07244   hnRNP K   mRNA transport   50.9   5.39   6   0.52   0.53   0.50   Unchanged∥ 
P07029   hnRNP UP2   mRNA splicing   24.1   8.02   1   1.0   0.68   ND   Unchanged∥ 
P43331   snRNP D3   mRNA splicing   13.9   10.3   2   ND   0.39   0.41   Unchanged∥25  
P08578   snRNP E   mRNA splicing   10.8   9.46   2   0.71   0.62   0.59   Unchanged∥ 
Q13822   E-NPP 2   Glycolysis   99.0   7.14   2   ND   ND   0.31   Unknown  
P22712   MBP-1   Transcription   37.0   6.79   2   ND   0.15   ND   Unknown  
Q9BWX5   GATA-5   Transcription   41.3   9.17   1   1.5   ND   2.6   Unknown  
Q14974   Importin β-1   Transport   97.2   4.68   2   1.5   ND   2.1   Repressed24  
P24903   CYPIIF1   Cellular defense   55.5   6.93   2   1.6   ND   1.8   Unknown  
P52565   Rho GDI 1   Signal transduction   23.2   5.03   2   2.3   1.7   ND   Unknown  
P31146   Coronin 1A   Signal transduction   51.0   6.25   3   2.2   ND   ND   Unknown  
NP_000994  60S P1 protein   Protein synthesis   11.5   4.26   2   1.0   1.5   1.8   Unknown  
P15531   NDP kinase A   Energy   17.1   5.83   2   0.9   1.5   ND   Unknown  
P05109   Calgranulin A   Cellular defense   10.8   6.51   2   ND   2.0   2.1   Induced23  
Q99733   NAP2   Not defined   42.7   4.60   3   1.2   ND   1.9   Unknown  
P47755   CapZ α-2   Structural   32.9   5.57   2   1.8   2.2   2.6   Unknown  
P12814   α-actinin 1   Structural   103   5.22   3   2.7   2.3   2.8   Unknown  
Q08043   α-actinin 3   Structural   103   5.40   3   3.4   ND   ND   Unknown  
P35579   NMMHC-A   Structural   226   5.50   3   2.0   4.1   4.3   Unknown  
P29590
 		 isoform PML-1
 		 Negative regulator
 		 97.5
 		 5.88
 		 2
 		 3.5
 		 ND
 		 ND
 		 Unknown
 
Peptides sequenced
Protein expression†
Accession no.
Protein name
Functional classification
Mr, kDa*
pl*
24 h
72 h
96 h
mRNA expression‡
P04720   eEF1A-1   Protein synthesis   50.1   9.10   5   0.67   0.32   0.36   Unchanged∥24  
P29692   EF-1-δ   Protein synthesis   31.1   4.90   2   0.80   0.53   ND   Unchanged24  
AAH09865  eEF1γ   Protein synthesis   50.1   6.25   4   0.35   0.41   0.38   Unchanged∥ 
P46199   IF-2   Protein synthesis   81.3   6.71   2   ND   0.47   0.35   Unchanged24  
P04765   eIF4A-1   Protein synthesis   46.1   5.32   3   0.53   0.34§  0.40§  Unchanged∥ 
P55010   eIF5   Protein synthesis   49.2   5.41   2   ND   0.48   ND   Unchanged24  
P42655   14-3-3ϵ   Signal transduction   29.1   4.63   4   0.66   0.44   0.41§  Unknown  
P29312   14-3-3ζ/δ   Signal transduction   27.7   4.73   2   0.81   0.58   0.59   Unknown  
P01112   c-H-ras   Signal transduction   21.2   5.16   2   ND   ND   0.19   Unknown  
P00540   c-mos   Signal transduction   37.8   9.15   3   ND   0.43   0.38   Unknown  
P30153   PP2A   Signal transduction   65.0   4.96   2   0.70   0.35   0.41   Unknown  
Q13233   MEKK 1   Signal transduction   164   8.52   3   0.92   ND   0.64   Repressed24  
P23528   Cofilin, P18   Structural   18.5   8.22   9   0.51   0.64   0.52   Repressed24  
P32119   Peroxiredoxin 2   Cellular defense   21.9   5.66   5   ND   0.47   0.50   Unknown  
C28894   MPO   Cellular defense   92.4   9.25   9   0.35   0.52   0.48   Repressed23  
P25763   CDC42 homolog   Not defined   21.3   6.16   3   0.35   0.54   ND   Repressed24  
P10809   60-kDa hsp   Chaperone   61.0   5.70   7   0.77   0.67   0.48   Repressed23  
Q9NR31   SAR1a   Transport   22.4   6.22   4   ND   0.18   0.20   Unknown  
P12270   Nucleoprotein TPR   mRNA transport   266   5.01   6   0.88   0.50   0.48   Unknown  
P07910   hnRNP C1/C2   mRNA splicing   33.7   4.95   3   0.64   0.55   ND   Unchanged∥ 
Q07244   hnRNP K   mRNA transport   50.9   5.39   6   0.52   0.53   0.50   Unchanged∥ 
P07029   hnRNP UP2   mRNA splicing   24.1   8.02   1   1.0   0.68   ND   Unchanged∥ 
P43331   snRNP D3   mRNA splicing   13.9   10.3   2   ND   0.39   0.41   Unchanged∥25  
P08578   snRNP E   mRNA splicing   10.8   9.46   2   0.71   0.62   0.59   Unchanged∥ 
Q13822   E-NPP 2   Glycolysis   99.0   7.14   2   ND   ND   0.31   Unknown  
P22712   MBP-1   Transcription   37.0   6.79   2   ND   0.15   ND   Unknown  
Q9BWX5   GATA-5   Transcription   41.3   9.17   1   1.5   ND   2.6   Unknown  
Q14974   Importin β-1   Transport   97.2   4.68   2   1.5   ND   2.1   Repressed24  
P24903   CYPIIF1   Cellular defense   55.5   6.93   2   1.6   ND   1.8   Unknown  
P52565   Rho GDI 1   Signal transduction   23.2   5.03   2   2.3   1.7   ND   Unknown  
P31146   Coronin 1A   Signal transduction   51.0   6.25   3   2.2   ND   ND   Unknown  
NP_000994  60S P1 protein   Protein synthesis   11.5   4.26   2   1.0   1.5   1.8   Unknown  
P15531   NDP kinase A   Energy   17.1   5.83   2   0.9   1.5   ND   Unknown  
P05109   Calgranulin A   Cellular defense   10.8   6.51   2   ND   2.0   2.1   Induced23  
Q99733   NAP2   Not defined   42.7   4.60   3   1.2   ND   1.9   Unknown  
P47755   CapZ α-2   Structural   32.9   5.57   2   1.8   2.2   2.6   Unknown  
P12814   α-actinin 1   Structural   103   5.22   3   2.7   2.3   2.8   Unknown  
Q08043   α-actinin 3   Structural   103   5.40   3   3.4   ND   ND   Unknown  
P35579   NMMHC-A   Structural   226   5.50   3   2.0   4.1   4.3   Unknown  
P29590
 		 isoform PML-1
 		 Negative regulator
 		 97.5
 		 5.88
 		 2
 		 3.5
 		 ND
 		 ND
 		 Unknown
 

eEF indicates eukaryotic translation elongation factor; c-H-ras, transforming protein p21/H-Ras-1; c-mos, proto-oncogene serine/threonine-protein kinase mos; PP2A, serine/threonine protein phosphatase 2A; MEKK 1, mitogen-activated protein kinase kinase kinase 1; MPO, myeloperoxidase; CDC42 homolog, G25K guanosine triphosphate (GTP)-binding protein; SAR1a, GTP-binding protein SAR1a; hnRNP, heterogeneous nuclear ribonucleoprotein; snRNP, small nuclear ribonucleoprotein; E-NPP 2, ectonucleotide pyrophosphatase/phosphodiesterase 2; MBP-1, C-myc promoter-binding protein; GATA-5, transcription factor GATA-5; CYPIIF1, cytochrome P450 2F1; NDP kinase A, nucleoside diphosphate kinase A; NAP2, nucleosome assembly protein 1—like 4; actinin, F-actin cross-linking protein; NMMHC-A, myosin heavy chain, nonmuscle type A; isoform PML-1, probable transcription factor PML. For other abbreviations, see Table 1.

*

Theoretical.

†

ATRA-treated divided by nontreated. All values are statistically significant, P < .05.

‡

The mRNA expression is as displayed in references Liu et al23  (for 0 to 72 hours), Yang et al24  (for 0 to 96 hours), or Lee et al25  (for 0 to 48 hours) or by personal communication. Unchanged mRNA expression ratios (for ATRA treated divided by nontreated) were within 0.8 to 1.2 as a function of various ATRA treatment time points.

∥

See Figure 4.

§

Also in Table 1.

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