Long-term cultivated ES-EPs exhibit a stable karyotype
. | No. of ES-EP cells . | . | |
|---|---|---|---|
| No. of chromosomes/cell . | 62 d . | 96 d . | |
| 38 | 1 | 1 | |
| 39 | 3 | 4 | |
| 40 | 140 | 126 | |
| 41 | 1 | 0 | |
| 42 | 0 | 0 | |
| 78 | 0 | 0 | |
| 79 | 0 | 0 | |
| 80 | 0 | 0 | |
| 81 | 0 | 0 | |
| 82 | 0 | 0 | |
| Total no. of counted cells | 144 | 133 | |
. | No. of ES-EP cells . | . | |
|---|---|---|---|
| No. of chromosomes/cell . | 62 d . | 96 d . | |
| 38 | 1 | 1 | |
| 39 | 3 | 4 | |
| 40 | 140 | 126 | |
| 41 | 1 | 0 | |
| 42 | 0 | 0 | |
| 78 | 0 | 0 | |
| 79 | 0 | 0 | |
| 80 | 0 | 0 | |
| 81 | 0 | 0 | |
| 82 | 0 | 0 | |
| Total no. of counted cells | 144 | 133 | |
Chromosome spreads of ES-EPs cultured for 62 days (n = 3) or 96 days (n = 2) reveal a diploid set of chromosomes in essentially all metaphases analyzed.