Marker expression of ES-EP cells at different time points of expansion from EBs
. | EB . | . | ES-EP . | . | . | . | FL-EP . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Marker . | Day 6, % . | Day 7, % . | Day 14, % . | Day 21, % . | Day 40, % . | Day 82, % . | Day 7, % . | Day 14, % . | |||||
| Multipotent | |||||||||||||
| Sca-1 | 8.6 ± 1.9 | 2.0 ± 0.7 | 1.1 ± 0.6 | < 1 | < 1 | < 0.1 | 4.8 ± 2.9 | 2.3 ± 2.4 | |||||
| CD34 | 5.5 ± 2.3 | 25.3 ± 7.7 | 15.9 ± 1.3 | 13.9 ± 0.1 | 8.8 ± 1.6 | < 1 | 11.6 ± 6.1 | 6.5 ± 2.1 | |||||
| CD117 | 61.2 ± 5 | 50.1 ± 10.0 | 45.6 ± 11 | 38.4 ± 2.3 | 64.0 ± 9.1 | 62.0 ± 7.7 | 59.5 ± 6.4 | 57.5 ± 3.5 | |||||
| Erythroid | |||||||||||||
| Ter119 | 2.9 ± 1.4 | 40.0 ± 4.4 | 75.6 ± 7.9 | 94.3 ± 2.4 | 88.5 ± 5.7 | 69.6 ± 14.8 | 42.2 ± 3.0 | 31.5 ± 5.5 | |||||
| [MFI of Ter119] | [13.9 ± 8.3] | [185.4 ± 81.5] | [191.7 ± 64.8] | [425.5 ± 120.9] | [175.0 ± 42.44] | [42.0 ± 1.4] | [28.6 ± 1.5] | [91.5 ± 33.4] | |||||
| Ter119/CD117 | 0.9 ± 0.3 | 35.0 ± 15.2 | 47.0 ± 5.7 | 37.9 ± 3.2 | 65.0 ± 23.5 | 13.9 ± 2.0 | 11.0 ± 2.8 | 13.0 ± 0.7 | |||||
| Myeloid | |||||||||||||
| Mac1 | < 0.1 | 3.7 ± 2.1 | < 1 | < 1 | < 0.1 | < 0.1 | 7.3 ± 4.0 | < 1 | |||||
| Lymphoid | |||||||||||||
| B220 | 1.0 ± 0.4 | < 1 | < 1 | < 0.1 | < 0.1 | < 0.1 | 1.2 ± 0.1 | < 1 | |||||
| CD19 | < 0.1 | < 1 | < 0.1 | < 0.1 | < 0.1 | < 0.1 | 2.2 ± 0.8 | < 1 | |||||
| CD4 | < 0.1 | < 1 | < 0.1 | < 0.1 | < 0.1 | < 0.1 | 2.4 ± 1.5 | < 1 | |||||
| Endothelial | |||||||||||||
| Flk-1 | 41.6 ± 8.7 | < 0.1 | < 0.1 | < 0.1 | < 0.1 | ND | 1.6 ± 0.6 | 1.1 ± 1.6 | |||||
| Proliferating cell marker | |||||||||||||
| CD71 | 91.0 | 98 ± 1.2 | 99.0 ± 0.8 | 99.8 ± 0.2 | 99.7 ± 0.3 | ND | 99.0 ± 1.4 | 99.2 ± 0.4 | |||||
. | EB . | . | ES-EP . | . | . | . | FL-EP . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Marker . | Day 6, % . | Day 7, % . | Day 14, % . | Day 21, % . | Day 40, % . | Day 82, % . | Day 7, % . | Day 14, % . | |||||
| Multipotent | |||||||||||||
| Sca-1 | 8.6 ± 1.9 | 2.0 ± 0.7 | 1.1 ± 0.6 | < 1 | < 1 | < 0.1 | 4.8 ± 2.9 | 2.3 ± 2.4 | |||||
| CD34 | 5.5 ± 2.3 | 25.3 ± 7.7 | 15.9 ± 1.3 | 13.9 ± 0.1 | 8.8 ± 1.6 | < 1 | 11.6 ± 6.1 | 6.5 ± 2.1 | |||||
| CD117 | 61.2 ± 5 | 50.1 ± 10.0 | 45.6 ± 11 | 38.4 ± 2.3 | 64.0 ± 9.1 | 62.0 ± 7.7 | 59.5 ± 6.4 | 57.5 ± 3.5 | |||||
| Erythroid | |||||||||||||
| Ter119 | 2.9 ± 1.4 | 40.0 ± 4.4 | 75.6 ± 7.9 | 94.3 ± 2.4 | 88.5 ± 5.7 | 69.6 ± 14.8 | 42.2 ± 3.0 | 31.5 ± 5.5 | |||||
| [MFI of Ter119] | [13.9 ± 8.3] | [185.4 ± 81.5] | [191.7 ± 64.8] | [425.5 ± 120.9] | [175.0 ± 42.44] | [42.0 ± 1.4] | [28.6 ± 1.5] | [91.5 ± 33.4] | |||||
| Ter119/CD117 | 0.9 ± 0.3 | 35.0 ± 15.2 | 47.0 ± 5.7 | 37.9 ± 3.2 | 65.0 ± 23.5 | 13.9 ± 2.0 | 11.0 ± 2.8 | 13.0 ± 0.7 | |||||
| Myeloid | |||||||||||||
| Mac1 | < 0.1 | 3.7 ± 2.1 | < 1 | < 1 | < 0.1 | < 0.1 | 7.3 ± 4.0 | < 1 | |||||
| Lymphoid | |||||||||||||
| B220 | 1.0 ± 0.4 | < 1 | < 1 | < 0.1 | < 0.1 | < 0.1 | 1.2 ± 0.1 | < 1 | |||||
| CD19 | < 0.1 | < 1 | < 0.1 | < 0.1 | < 0.1 | < 0.1 | 2.2 ± 0.8 | < 1 | |||||
| CD4 | < 0.1 | < 1 | < 0.1 | < 0.1 | < 0.1 | < 0.1 | 2.4 ± 1.5 | < 1 | |||||
| Endothelial | |||||||||||||
| Flk-1 | 41.6 ± 8.7 | < 0.1 | < 0.1 | < 0.1 | < 0.1 | ND | 1.6 ± 0.6 | 1.1 ± 1.6 | |||||
| Proliferating cell marker | |||||||||||||
| CD71 | 91.0 | 98 ± 1.2 | 99.0 ± 0.8 | 99.8 ± 0.2 | 99.7 ± 0.3 | ND | 99.0 ± 1.4 | 99.2 ± 0.4 | |||||
Freshly dissociated cells from embryoid bodies (EBs) day 6, erythroid progenitors from mouse ES cells (ES-EPs) cultured between 7 days and 82 days and as a control, fetal liver-derived erythroid progenitors (FL-EPs) after 7 days and 14 days of cultivation were analyzed by flow cytometry for the expression of a panel of hematopoietic and endothelial lineage markers. For Ter119, the mean fluorescent intensity (MFI) is also plotted. Mean values and standard deviation were calculated from at least 3 independent determinations.
ND indicates not determined.