Quantitation of K562-MERT colonies and cells in methylcellulose cultures
Treatment* . | No. of colonies† . | No. of cells‡ . |
|---|---|---|
| None | 840 ± 198 | 1.70 ± 0.223 × 105 |
| NmU | 780 ± 201 | 5.21 ± 0.303 × 105 |
| NmU + 4-OHT | 117 ± 61 | 1.06 ± 0.597 × 105 |
| 4-OHT | 63 ± 24 | 0.443 ± 0.138 × 105 |
Treatment* . | No. of colonies† . | No. of cells‡ . |
|---|---|---|
| None | 840 ± 198 | 1.70 ± 0.223 × 105 |
| NmU | 780 ± 201 | 5.21 ± 0.303 × 105 |
| NmU + 4-OHT | 117 ± 61 | 1.06 ± 0.597 × 105 |
| 4-OHT | 63 ± 24 | 0.443 ± 0.138 × 105 |
K562-MERT cells were combined with a cytokine cocktail and methylcellulose as described in “Materials and methods.” In addition, the methylcellulose cultures received either (1) “none,” which is no additional reagents; (2) “NmU,” which is the addition of 35 μM NmU peptide; (3) “NmU + 4-OHT,” which is the addition of both 35 μM NmU peptide and 1 μM 4-OHT; or (4) “4-OHT,” which is the addition of 1 μM 4-OHT.
K562-MERT colonies were scored on day 14. The number of K562-MERT colonies in each experimental condition is the mean of 8 individual determinations ± SD.
The total K562-MERT colonies per treatment were dissociated into individual cells with warm PBS and counted in a hemacytometer. The number of K562-MERT cells in each experimental condition is the mean of 4 individual determinations ± SD.