Relationship between absolute RHAMM expression, at the message and protein level, and RHAMM isoform ratio in mitotic and unsynchronized cell lines
. | Quantitative RT-PCR* . | Quantitative immunobiol* . | RHAMM ratio . |
|---|---|---|---|
| HeLa | |||
| Unsynchronized | 1.0 | 1.0 | 0.48 ± 0.06 |
| Mitotic | 1.74 ± 0.08 | 1.62 | 0.56 ± 0.04 |
| Raji | |||
| Unsynchronized | 1.0 | 1.0 | 0.61 ± 0.05 |
| Mitotic | 1.43 ± 0.08 | 1.51 | 0.57 ± 0.03 |
. | Quantitative RT-PCR* . | Quantitative immunobiol* . | RHAMM ratio . |
|---|---|---|---|
| HeLa | |||
| Unsynchronized | 1.0 | 1.0 | 0.48 ± 0.06 |
| Mitotic | 1.74 ± 0.08 | 1.62 | 0.56 ± 0.04 |
| Raji | |||
| Unsynchronized | 1.0 | 1.0 | 0.61 ± 0.05 |
| Mitotic | 1.43 ± 0.08 | 1.51 | 0.57 ± 0.03 |
HeLa and Raji cells were synchronized by double thymidine and nocodazole block. Quantitative RT-PCR was performed as described. Quantitative immunoblot analysis was performed on CHAPS+ soluble lysates using infrared detection and Odyssey imaging as described. Band quantitation was normalized to B-actin levels.
RHAMM ratios were determined as described.
Data included in Maxwell et al, submitted.