Table 1. Previously activated MoDCs... | American Society of Hematology

Table 1.

Previously activated MoDCs represent irreversibly committed mature DC populations

Initial activation protocol	IL-6, ng/mL	IL-12p70, pg/mL
Plus E coli 24 h after activation*
No cytokines	104 ± 32	3046 ± 289
GM+T+N+P	0	34 ± 34
CD40L₃+IFN-γ	14 ± 10	901 ± 150
CD40L₁+IFN-γ	3.8 ± 2.5	453 ± 155
*Plus E coli* following 24 h of activation plus 24 h of rest†**
No cytokines	200 ± 0	497 ± 138
GM+T+N+P	4.4 ± 3	0
CD40L₃+IFN-γ	73 ± 42	0
CD40L₁+IFN-γ	63 ± 46	0

Initial activation protocol	IL-6, ng/mL	IL-12p70, pg/mL
Plus E coli 24 h after activation*
No cytokines	104 ± 32	3046 ± 289
GM+T+N+P	0	34 ± 34
CD40L₃+IFN-γ	14 ± 10	901 ± 150
CD40L₁+IFN-γ	3.8 ± 2.5	453 ± 155
*Plus E coli* following 24 h of activation plus 24 h of rest†**
No cytokines	200 ± 0	497 ± 138
GM+T+N+P	4.4 ± 3	0
CD40L₃+IFN-γ	73 ± 42	0
CD40L₁+IFN-γ	63 ± 46	0

Cytokine secretion by MoDCs initially activated with GM-CSF plus TNF-α plus IFN-α plus PGE₂ (GM+T+N+P), CD40L₁ plus IFN-γ, or CD40L₃ plus IFN-γ (protocols as in the legend to Figure 1). Twenty-four hours after activation, DCs were washed twice and resuspended in RPMI/FCS. The indicated activation stimuli (no cytokines or live E coli [20 μL/mL]) were added either immediately (*) or following a 24-hour rest period in medium without cytokines (†). Mean values ± SEM of 4 experiments are shown.

View Large

This Feature Is Available To Subscribers Only