Table 1. Amphoterin is not chemotactic... | American Society of Hematology

Table 1.

Amphoterin is not chemotactic for monocytes

	Endothelial cell monolayer, %	Polycarbonate filter, %
Noninduced	100	100
Amphoterin	98.9 ± 15.9	88.9 ± 24.4
fMLP	681.2 ± 123.2^*	152.0 ± 13.3^*

	Endothelial cell monolayer, %	Polycarbonate filter, %
Noninduced	100	100
Amphoterin	98.9 ± 15.9	88.9 ± 24.4
fMLP	681.2 ± 123.2^*	152.0 ± 13.3^*

Monocyte migration toward recAtn or fMLP was studied in Transwell chamber assay. The lower compartment containing chemotactic stimuli and the upper compartment containing monocytes were separated by either the endothelial monolayer-coated porous polycarbonate filter or -uncoated porous polycarbonate filter. Amphoterin and fMLP concentrations in the endothelial cell monolayer assay were 5 nM. Amphoterin and fMLP concentrations in the polycarbonate filter assay were 40 nM and 100 nM, respectively. Cells were allowed to migrate for 3 hours, and the number of transmigrated cells was determined (n = 3). ^*P < .05.

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