Table 2. Tetramer, IFN-γ T-cell... | American Society of Hematology

Table 2.

Tetramer, IFN-γ T-cell frequency, and CRA results of PBMCs before and after IVS

	% Tetramer binding					% IFN-γ
UPN	IE1	pp65	Nonrelated	IE1	pp65	Nonrelated	IE1	pp65	Nonrelated
009
PBMC^*	6.89	2.90	0.38	4.60	2.97	0.19	34.89	19.03	11.56
After IVS†	72.26	32.30	0.13	16.70	7.40	0.22	99.19	69.05	9.91
010
PBMC	2.30	8.45	0.50	1.60	1.10	0.02	8.73	26.51	5.32
After IVS	9.60	30.19	0.17	11.34	21.47	0.93	71.25	88.34	28.1
011
PBMC	0.37	3.00	0.08	0.45	2.00	0.05	3.64	9.68	0.01
After IVS	3.91	57.44	0.41	1.73	17.17	0.21	61.58	97.71	22.37

	% Tetramer binding					% IFN-γ
UPN	IE1	pp65	Nonrelated	IE1	pp65	Nonrelated	IE1	pp65	Nonrelated
009
PBMC^*	6.89	2.90	0.38	4.60	2.97	0.19	34.89	19.03	11.56
After IVS†	72.26	32.30	0.13	16.70	7.40	0.22	99.19	69.05	9.91
010
PBMC	2.30	8.45	0.50	1.60	1.10	0.02	8.73	26.51	5.32
After IVS	9.60	30.19	0.17	11.34	21.47	0.93	71.25	88.34	28.1
011
PBMC	0.37	3.00	0.08	0.45	2.00	0.05	3.64	9.68	0.01
After IVS	3.91	57.44	0.41	1.73	17.17	0.21	61.58	97.71	22.37

Combined exposure of PBMCs to pp65/pp150-MVA and IE4-MVA was carried out as described in “Materials and methods.” Immunity to IE1_316-324, pp65_495-503, and nonrelated peptide p53_149-157 was determined for HLA A^*0201 donors 009, 010, and 011, as specified in “Materials and methods,” and listed as percentages. CRA killing is reported at E/T 100 for PBMCs and at E/T 20 for post-IVS cultures.

PBMC = fresh uncultured PBMC

†

After IVS = cultured cells after IVS

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