CD80 and CD86 expression by BCP-ALL cells activated or not activated with CD40L
. | Positive cells, % . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | CD80 . | . | CD86 . | . | |||
| Patient . | -CD40L . | +CD40L . | -CD40L . | +CD40L . | |||
| 1 | 3 | 10 | 7 | 15 | |||
| 2 | 0 | 0 | 0 | 10 | |||
| 3 | 5 | 25 | 34 | 55 | |||
| 4 | 0 | 0 | 80 | 90 | |||
| 5 | 9 | 20 | 64 | 78 | |||
| 6 | 0 | 20 | 20 | 40 | |||
| 7 | 0 | 5 | 50 | 60 | |||
| 8 | 18 | 44 | 27 | 45 | |||
| 9 | 0 | 0 | 0 | 5 | |||
| 10 | 15 | 52 | 30 | 70 | |||
| 11 | 50 | 70 | 60 | 70 | |||
| 12 | 20 | 68 | 22 | 68 | |||
| 13 | 30 | 40 | 58 | 70 | |||
| 14 | 0 | 8 | 10 | 35 | |||
| 15 | 70 | 90 | 70 | 94 | |||
| 16 | 30 | 30 | 60 | 72 | |||
| 17 | 0 | 15 | 20 | 40 | |||
| 18 | 0 | 0 | 0 | 5 | |||
| 19 | 0 | 30 | 25 | 60 | |||
. | Positive cells, % . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | CD80 . | . | CD86 . | . | |||
| Patient . | -CD40L . | +CD40L . | -CD40L . | +CD40L . | |||
| 1 | 3 | 10 | 7 | 15 | |||
| 2 | 0 | 0 | 0 | 10 | |||
| 3 | 5 | 25 | 34 | 55 | |||
| 4 | 0 | 0 | 80 | 90 | |||
| 5 | 9 | 20 | 64 | 78 | |||
| 6 | 0 | 20 | 20 | 40 | |||
| 7 | 0 | 5 | 50 | 60 | |||
| 8 | 18 | 44 | 27 | 45 | |||
| 9 | 0 | 0 | 0 | 5 | |||
| 10 | 15 | 52 | 30 | 70 | |||
| 11 | 50 | 70 | 60 | 70 | |||
| 12 | 20 | 68 | 22 | 68 | |||
| 13 | 30 | 40 | 58 | 70 | |||
| 14 | 0 | 8 | 10 | 35 | |||
| 15 | 70 | 90 | 70 | 94 | |||
| 16 | 30 | 30 | 60 | 72 | |||
| 17 | 0 | 15 | 20 | 40 | |||
| 18 | 0 | 0 | 0 | 5 | |||
| 19 | 0 | 30 | 25 | 60 | |||
BCP-ALL cells were cocultured on HBMS in the presence or absence of CD40L transfectants. After 48 hours, the cells were stained with FITC-conjugated mAbs. Phenotypic analysis was performed on the gated cells to exclude cell debris. Results are expressed as percentage of positive cells.