Differential binding of BMNCs to fucoidan
Cell type . | Input, % . | Bound, % . | P . |
|---|---|---|---|
| Erythroid, TER119+ | 12.3 ± 1.2 | 2.2 ± 0.5 | .0004 |
| B-lymphoid, B220+ | 34.3 ± 3.2 | 28.2 ± 1.6 | .1305 |
| Myeloid, Mac-1+ | 52.1 ± 0.8 | 72.9 ± 0.6 | .0045 |
Cell type . | Input, % . | Bound, % . | P . |
|---|---|---|---|
| Erythroid, TER119+ | 12.3 ± 1.2 | 2.2 ± 0.5 | .0004 |
| B-lymphoid, B220+ | 34.3 ± 3.2 | 28.2 ± 1.6 | .1305 |
| Myeloid, Mac-1+ | 52.1 ± 0.8 | 72.9 ± 0.6 | .0045 |
BMNCs from wild-type mice were allowed to adhere to immobilized fucoidan for 30 minutes. Bound cells were detached with trypsin/EDTA and stained with the antibodies TER119, B220, and M1/70 to stain for cells of the erythroid, lymphoid, and myeloid lineages, respectively. Input BMNCs used for the experiment were also stained for comparison. Data are expressed as mean percentages ± SEM from 3 to 5 mice in 3 independent experiments. Note that only the myeloid population is significantly enriched in the bound population while the erythroid population is depleted.