Clinical diagnostic tests developed from genome-wide gene expression data
Diagnostic methodology . | Advantages . | Disadvantages . | No. of features . |
|---|---|---|---|
| Whole genome microarray | Inclusive, universal platform for all applications | Expensive, complex data output | 1000s |
| Custom microarray | Allows assessment of many genes | Biased in only assessing selected genes | 10s to 100s |
| Other gene expression platforms: bead or fiberoptic based | Allows assessment of many genes | Requires further validation | 10s to 100s |
| Antibody microarray | Allows assessment of many proteins | Requires set of specific antibodies | 10s to 100s |
| Quantitative RT-PCR | Potential to analyze paraffin-embedded specimens | Challenging to multiplex | 10s |
| Flow cytometry | Routinely available | Limited to cell surface markers | 1 to several |
| Immunohistochemistry | Routinely available, spatial localization, assess morphology | Nonquantitative | 1 to several |
Diagnostic methodology . | Advantages . | Disadvantages . | No. of features . |
|---|---|---|---|
| Whole genome microarray | Inclusive, universal platform for all applications | Expensive, complex data output | 1000s |
| Custom microarray | Allows assessment of many genes | Biased in only assessing selected genes | 10s to 100s |
| Other gene expression platforms: bead or fiberoptic based | Allows assessment of many genes | Requires further validation | 10s to 100s |
| Antibody microarray | Allows assessment of many proteins | Requires set of specific antibodies | 10s to 100s |
| Quantitative RT-PCR | Potential to analyze paraffin-embedded specimens | Challenging to multiplex | 10s |
| Flow cytometry | Routinely available | Limited to cell surface markers | 1 to several |
| Immunohistochemistry | Routinely available, spatial localization, assess morphology | Nonquantitative | 1 to several |