Cell-cycle analysis of HCD57 cells treated with SP600125 inhibitor for 24 hours
. | EPO . | EPO + vehicle . | EPO + 12.5 μM SP600125 . | EPO + 25 μM SP600125 . | No EPO . |
|---|---|---|---|---|---|
| Apoptotic | 6.2 ± 0.5 | 6.1 ± 0.7 | 10.9 ± 0.2 | 24.3 ± 2.5 | 74.4 ± 1.1 |
| G0/G1 | 39.6 ± 0.6 | 39.4 ± 0.2 | 32.0 ± 0.9 | 4.7 ± 2.2 | 10.9 ± 0.6 |
| S phase | 20.9 ± 0.3 | 20.7 ± 0.4 | 15.2 ± 1.0 | 3.8 ± 1.8 | 6.0 ± 0.8 |
| G2/M | 38.6 ± 1.4 | 38.4 ± 0.1 | 46.1 ± 0.2 | 77.0 ± 0.4 | 9.20 ± 0.26 |
. | EPO . | EPO + vehicle . | EPO + 12.5 μM SP600125 . | EPO + 25 μM SP600125 . | No EPO . |
|---|---|---|---|---|---|
| Apoptotic | 6.2 ± 0.5 | 6.1 ± 0.7 | 10.9 ± 0.2 | 24.3 ± 2.5 | 74.4 ± 1.1 |
| G0/G1 | 39.6 ± 0.6 | 39.4 ± 0.2 | 32.0 ± 0.9 | 4.7 ± 2.2 | 10.9 ± 0.6 |
| S phase | 20.9 ± 0.3 | 20.7 ± 0.4 | 15.2 ± 1.0 | 3.8 ± 1.8 | 6.0 ± 0.8 |
| G2/M | 38.6 ± 1.4 | 38.4 ± 0.1 | 46.1 ± 0.2 | 77.0 ± 0.4 | 9.20 ± 0.26 |
Values presented are percentage of cells in each phase of cell cycle. Phase of cell cycle was determined by flow cytometry analysis of propidium iodide—stained cells. Error indicates standard deviation of 3 independent samples.