Recognition of target cells expressing CMV antigens by CD8+ CTL clones isolated directly from the blood of CMV-seropositive donors
. | Clones that lyse target cells infected with . | . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor no. (no. of clones) . | RV798, no. (%) . | AD169, no. (%) . | Vac/IE, no. (%) . | Vac/pp65, no. (%) . | Vac/pp150, no. (%) . | Vac/gB, no. (%) . | Unknown specificity, no. (%) . | |||||
| 1 (87) | 87 (100) | 46 (53) | 16 (18) | 46 (53) | 0 (0) | 0 (0) | 25 (29) | |||||
| 2 (74) | 74 (100) | 37 (50) | 0 (0) | 37 (50) | 0 (0) | 4 (5) | 33 (45) | |||||
| 3 (54) | 54 (100) | 34 (63) | 4 (7) | 18 (33) | 16 (30) | 0 (0) | 16 (30) | |||||
| 5 (87) | 87 (100) | 15 (17) | 3 (3) | 12 (14) | 3 (3) | 0 (0) | 69 (80) | |||||
| 7 (83) | 83 (100) | 20 (24) | 23 (28) | 20 (24) | 0 (0) | 3 (4) | 37 (44) | |||||
| Total (385) | 385 (100) | 152 (39) | 46 (12) | 133 (34) | 19 (5) | 7 (2) | 180 (47) | |||||
. | Clones that lyse target cells infected with . | . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor no. (no. of clones) . | RV798, no. (%) . | AD169, no. (%) . | Vac/IE, no. (%) . | Vac/pp65, no. (%) . | Vac/pp150, no. (%) . | Vac/gB, no. (%) . | Unknown specificity, no. (%) . | |||||
| 1 (87) | 87 (100) | 46 (53) | 16 (18) | 46 (53) | 0 (0) | 0 (0) | 25 (29) | |||||
| 2 (74) | 74 (100) | 37 (50) | 0 (0) | 37 (50) | 0 (0) | 4 (5) | 33 (45) | |||||
| 3 (54) | 54 (100) | 34 (63) | 4 (7) | 18 (33) | 16 (30) | 0 (0) | 16 (30) | |||||
| 5 (87) | 87 (100) | 15 (17) | 3 (3) | 12 (14) | 3 (3) | 0 (0) | 69 (80) | |||||
| 7 (83) | 83 (100) | 20 (24) | 23 (28) | 20 (24) | 0 (0) | 3 (4) | 37 (44) | |||||
| Total (385) | 385 (100) | 152 (39) | 46 (12) | 133 (34) | 19 (5) | 7 (2) | 180 (47) | |||||
Autologous fibroblasts were infected with RV798 for 48 hours or AD169 for 16 hours and used as target cells in a chromium release assay. Aliquots of autologous LCLs were infected with each of the vaccinia CMV recombinant viruses for 12 hours. Clones categorized as lysing RV798-infected target cells exhibited greater than 20% lysis of RV798-infected cells and less than 3% lysis of mock-infected cells. Clones categorized as lysing AD169-infected target cells exhibited greater than 10% lysis of AD169-infected cells and less than 3% lysis of mock-infected cells. Clones were categorized as specific for a CMV antigen expressed by a vaccinia recombinant virus if they exhibited greater than 20% lysis of cells infected with that vaccinia recombinant and less than 5% lysis of cells infected with all the other vaccinia recombinant viruses. The effector-to-target cell ratio in all experiments was 10:1, and the data are derived from duplicate experiments with triplicate samples in each assay.