Comparison of intracellular TNF-α and IL-12-secreting activities of CD33+ PBMCs after stimulation with LPS in patients treated (n = 5) or not (n = 5) with G-CSF
. | Median % of CD33+ PBMC (range) . | . | . | |
|---|---|---|---|---|
. | NIL . | G-CSF . | P . | |
| TNF-α | 62.42 (82.7-27.1) | 48.35 (41.8-78.6) | NS | |
| IL-12 | 38.55 (12.8-54.3) | 9.07 (0.1-12.9) | .02 | |
. | Median % of CD33+ PBMC (range) . | . | . | |
|---|---|---|---|---|
. | NIL . | G-CSF . | P . | |
| TNF-α | 62.42 (82.7-27.1) | 48.35 (41.8-78.6) | NS | |
| IL-12 | 38.55 (12.8-54.3) | 9.07 (0.1-12.9) | .02 | |
Whole PBMCs obtained from patients at the WBC zenith point were cultured with complete medium and stimulated with LPS, as described in “Patients, materials, and methods,” and stained for CD33+ surface antigen expression and intracellular TNF-α and IL-12 p40/p70 heterodimer. Data refer to percentages of myeloid CD33+ mononuclear cells, as shown in Figure 6. Data were compared by nonparametric Mann-Whitney test.
NS indicates not significant.