Table 1. T2-HLA-A*0201 binding affinity... | American Society of Hematology

Table 1.

T2-HLA-A*0201 binding affinity of SARS-Spike protein-derived peptides

Rank	Start position	Sequence	Score*	FI†
1	1042	VVFLHVTYV	484.238	0.5
2	982	RLQSLQTYV	382.536	0.3
3	958	VLNDILSRL	342.461	0.6
4	897	VLYENQKQI	232.693	0.4
5	1174	NLNESLIDL	201.447	1.1
6	734	LLLQYGSFC	171.868	0.4
7	411	KLPDDFMGC	135.453	0.4
8	787	ILPDPLKPT	119.463	0.3
9	151	MIFDNAFNC	102.176	0.4
10	2	FIFLLFLTL	94.987	-0.1
11	596	VLYQDVNCT	93.239	0.4
12	1096	IITTDNTFV	89.418	0.3
13	1167	RLNEVAKNL	87.586	1.7
14	846	LLTDDMIAA	79.642	0.4
15	735	LLQYGSFCT	73.477	0.2
16	1214	ILLCCMTSC	71.872	0.3
17	803	LLFNKVTLA	71.872	0.3
18	106	TMNNKSQSV	50.232	0.2
19	940	ALNTLVKQL	49.134	0.3
20	131	ELCDNPFFA	48.732	0.4

Rank	Start position	Sequence	Score*	FI†
1	1042	VVFLHVTYV	484.238	0.5
2	982	RLQSLQTYV	382.536	0.3
3	958	VLNDILSRL	342.461	0.6
4	897	VLYENQKQI	232.693	0.4
5	1174	NLNESLIDL	201.447	1.1
6	734	LLLQYGSFC	171.868	0.4
7	411	KLPDDFMGC	135.453	0.4
8	787	ILPDPLKPT	119.463	0.3
9	151	MIFDNAFNC	102.176	0.4
10	2	FIFLLFLTL	94.987	-0.1
11	596	VLYQDVNCT	93.239	0.4
12	1096	IITTDNTFV	89.418	0.3
13	1167	RLNEVAKNL	87.586	1.7
14	846	LLTDDMIAA	79.642	0.4
15	735	LLQYGSFCT	73.477	0.2
16	1214	ILLCCMTSC	71.872	0.3
17	803	LLFNKVTLA	71.872	0.3
18	106	TMNNKSQSV	50.232	0.2
19	940	ALNTLVKQL	49.134	0.3
20	131	ELCDNPFFA	48.732	0.4

Estimated half-time of dissociation (T_1/2) of HLA-A*0201 peptide complexes calculated using the website http://bimas.dcrt.nih.gov/molbio/hla_bind/index.html.

†

Increase of HLA-A*0201 molecules on T2 cells. FI = (mean FITC fluorescence with the given peptide-mean FITC fluorescence without peptide)/(mean FITC fluorescence without peptide). FI more than 1 indicates high-affinity peptides; FI of 1 or less, low-affinity peptides. Positive control peptide CAP-1 (YLSGANLNL) and negative control peptide OVA_257-264 (SIINFKEL) had FI 1.9 and 0.1, respectively.

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