Primers for RT-PCR experiments
Gene . | . | Sequence . | Nucleotide no. . |
|---|---|---|---|
| Chicken GAPDH | Sense | CCTCTCTGGCAAAGTCCAAG | 63-82 |
| Antisense | CATCCACCGTCTTCTGTGTG | 543-562 | |
| Chicken Flk1 | Sense | CAAGTGGGAGTTTCCCAGAG | 6-25 |
| Antisense | TTTGTGCAGGCTCCAAGTAA | 232-251 | |
| Chicken αSMA | Sense | GAATCCCAAAGCCAATCGAG | 336-355 |
| Antisense | AGGAGGAAGAGGCAGCAGT | 691-709 | |
| Chicken Ang-2 | Sense | GAGCAGACCCGCAAATTAAC | 142-161 |
| Antisense | GAACTGTCCAACCTCCTCCA | 687-706 | |
| Mouse GAPDH | Sense | ACCTTGTCAGCCTTTGCACT | 779-798 |
| Antisense | GCTGAAATCAGCACCGTGTA | 1259-1278 | |
| Mouse Flk1 | Sense | GATCACCATTCATCGCCTCT | 371-390 |
| Antisense | AAACATCTTCGCCACAGTCC | 851-870 | |
| Mouse PECAM-1 | Sense | CACCTCGAAAAGCAGGTCTC | 159-178 |
| Antisense | TGCACGGTGACGTATTCACT | 637-656 | |
| Mouse Endoglin | Sense | CACAACAGGTCTCGCAGAAA | 63-82 |
| Antisense | TTCTGGCAAGCACAAGAATG | 542-561 | |
| Mouse αSMA | Sense | TGTGCTGGACTCTGGAGATG | 459-478 |
| Antisense | CTTCTGCATCCTGTCAGCAA | 932-951 | |
| Mouse Ang-1 | Sense | ACCTTGTCAGCCTTTGCACT | 779-798 |
| Antisense | GCTGAAATCAGCACCGTGTA | 1259-1278 | |
| Mouse Ang-2 | Sense | CAAGTCAGGACTCACCACCA | 864-883 |
| Antisense | AAGTTGGAAGGACCACATGC | 1345-1364 | |
| Mouse Tie2 | Sense | GTGAAGCCAGATGGGACAGT | 1156-1175 |
| Antisense | TTGGCAGGAGACTGAGACCT | 1635-1654 | |
| Human GAPDH | Sense | ACCCAGAAGACTGTGGATGG | 610-629 |
| Antisense | CCCTGTTGCTGTAGCCAAAT | 1011-1030 | |
| Human Flk1 | Sense | CAGCTTCCAAGTGGCTAAGG | 3024-3042 |
| Antisense | ATTTCCCAAATGTTCCACCA | 3467-3486 | |
| Human αSMA | Sense | CTGAGCGTGGCTATTCCTTC | 587-606 |
| Antisense | GCTGGAAGGTGGACAGAGAG | 1047-1066 | |
| Human Ang-2 | Sense | CCACAAATGGCATCTACACG | 878-897 |
| Antisense | AAGTTGGAAGGACCACATGC | 1345-1364 |
Gene . | . | Sequence . | Nucleotide no. . |
|---|---|---|---|
| Chicken GAPDH | Sense | CCTCTCTGGCAAAGTCCAAG | 63-82 |
| Antisense | CATCCACCGTCTTCTGTGTG | 543-562 | |
| Chicken Flk1 | Sense | CAAGTGGGAGTTTCCCAGAG | 6-25 |
| Antisense | TTTGTGCAGGCTCCAAGTAA | 232-251 | |
| Chicken αSMA | Sense | GAATCCCAAAGCCAATCGAG | 336-355 |
| Antisense | AGGAGGAAGAGGCAGCAGT | 691-709 | |
| Chicken Ang-2 | Sense | GAGCAGACCCGCAAATTAAC | 142-161 |
| Antisense | GAACTGTCCAACCTCCTCCA | 687-706 | |
| Mouse GAPDH | Sense | ACCTTGTCAGCCTTTGCACT | 779-798 |
| Antisense | GCTGAAATCAGCACCGTGTA | 1259-1278 | |
| Mouse Flk1 | Sense | GATCACCATTCATCGCCTCT | 371-390 |
| Antisense | AAACATCTTCGCCACAGTCC | 851-870 | |
| Mouse PECAM-1 | Sense | CACCTCGAAAAGCAGGTCTC | 159-178 |
| Antisense | TGCACGGTGACGTATTCACT | 637-656 | |
| Mouse Endoglin | Sense | CACAACAGGTCTCGCAGAAA | 63-82 |
| Antisense | TTCTGGCAAGCACAAGAATG | 542-561 | |
| Mouse αSMA | Sense | TGTGCTGGACTCTGGAGATG | 459-478 |
| Antisense | CTTCTGCATCCTGTCAGCAA | 932-951 | |
| Mouse Ang-1 | Sense | ACCTTGTCAGCCTTTGCACT | 779-798 |
| Antisense | GCTGAAATCAGCACCGTGTA | 1259-1278 | |
| Mouse Ang-2 | Sense | CAAGTCAGGACTCACCACCA | 864-883 |
| Antisense | AAGTTGGAAGGACCACATGC | 1345-1364 | |
| Mouse Tie2 | Sense | GTGAAGCCAGATGGGACAGT | 1156-1175 |
| Antisense | TTGGCAGGAGACTGAGACCT | 1635-1654 | |
| Human GAPDH | Sense | ACCCAGAAGACTGTGGATGG | 610-629 |
| Antisense | CCCTGTTGCTGTAGCCAAAT | 1011-1030 | |
| Human Flk1 | Sense | CAGCTTCCAAGTGGCTAAGG | 3024-3042 |
| Antisense | ATTTCCCAAATGTTCCACCA | 3467-3486 | |
| Human αSMA | Sense | CTGAGCGTGGCTATTCCTTC | 587-606 |
| Antisense | GCTGGAAGGTGGACAGAGAG | 1047-1066 | |
| Human Ang-2 | Sense | CCACAAATGGCATCTACACG | 878-897 |
| Antisense | AAGTTGGAAGGACCACATGC | 1345-1364 |