Table 1.

Enrichment of mHag-specific polyclonal CTL lines by aAPCs in the absence or presence of additional autologous feeder cells or by DCs

Day 7
CTL lines
Day 0
aAPCs
aAPCs + PBMCs
aAPCs + CD4+Th
DCs
1      
HA-1A2 tetramer*  9.3   11.1   18.2   15.5   16.3  
Lysis: HA-1+/HA-1-†  68/26   37/20   59/25   38/11   48/22  
2      
HA-1A2 tetramer   9   11   19.6   22.2   13.9  
Lysis: HA-1+/HA-1-  51/-1   29/4   25/-3   36/3   21/1  
3      
HA-1A2 tetramer   5.3   8.7   8.6   16.2   13.8  
Lysis: HA-1+/HA-1-  43/5   19/-1   24/5   32/0   52/1  
4      
HA-1A2 tetramer   3.9   2.3   7.5   10.6   9.7  
Lysis: HA-1+/HA-1-
 		 40/5
 		 13/9
 		 45/19
 		 47/9
 		 53/7
 
Day 7
CTL lines
Day 0
aAPCs
aAPCs + PBMCs
aAPCs + CD4+Th
DCs
1      
HA-1A2 tetramer*  9.3   11.1   18.2   15.5   16.3  
Lysis: HA-1+/HA-1-†  68/26   37/20   59/25   38/11   48/22  
2      
HA-1A2 tetramer   9   11   19.6   22.2   13.9  
Lysis: HA-1+/HA-1-  51/-1   29/4   25/-3   36/3   21/1  
3      
HA-1A2 tetramer   5.3   8.7   8.6   16.2   13.8  
Lysis: HA-1+/HA-1-  43/5   19/-1   24/5   32/0   52/1  
4      
HA-1A2 tetramer   3.9   2.3   7.5   10.6   9.7  
Lysis: HA-1+/HA-1-
 		 40/5
 		 13/9
 		 45/19
 		 47/9
 		 53/7
 

The mHag-specific polyclonal CTL lines were expanded for 7 days in the presence of 20 U/mL IL-2 and 10 ng/mL IL-7. Responder-stimulator ratios were as follows: CTLs/aAPCs, 1:1; CTLs/aAPCs/PBMCs, 1:1:1; CTLs/aAPCs/CD4+Th, 5:5:1; CTLs/DCs, 10:1.

*

HA-1A2 tetramer—positive cells indicate the percentage of viable CD8+ HA-1A2 tetramer—positive lymphocytes in the total population of viable CD8+ lymphocytes.

†

Lysis indicates the percent lysis of HLA-A2+/HA-1+ or HLA-A2+/HA-1- EBV-LCLs; results are shown for an effector-target ratio of 10:1.

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