Sequences of primers and PCR conditions
Genes . | Primers . | Annealing temperature, °C . | Cycles . |
|---|---|---|---|
| BCR/ABL | Sense, 5′-AGCATTCCGCTGACCATCA-3′ | 62 | 30 |
| Antisense, 5′-AGCGGCTTCACTCAGACCC-3′ | |||
| ICSBP | Sense, 5′-AGAGGGAGACAAAGCTGAACC-3′ | 59 | 30 |
| Antisense, 5′-TGAATGGTGTGTGTCATAGGC-3′ | |||
| G-CSFR | Sense, 5′-CTCAAACCTATCCTGCCTCATG-3′ | 60 | 35 |
| Antisense, 5′-TCCAGGCAGAGATGAGCGAATG-3′ | |||
| c-fms | Sense, 5′-GCGATGTGTGAGCAATGGCAGT-3′ | 62 | 35 |
| Antisense, 5′-AGACCGTTTTGCGTAAGACCTG-3′ | |||
| GM-CSFR-α | Sense, 5′-GAGGTCCACAGGTCAAGGTG-3′ | 60 | 35 |
| Antisense, 5′-GATTGACAGTGGCAGGCTTC-3′ | |||
| GM-CSFR-β | Sense, 5′-TTTCCATCACAAACGAAGACT-3′ | 60 | 35 |
| Antisense, 5′-AATGAATGAGTAAGCCATCTT-3′ | |||
| PU.1 | Sense, 5′-TCTGATGGAGAAGCTGATGG-3′ | 55 | 31 |
| Antisense, 5′-CTTGACTTTCTTCACCTCGC-3′ | |||
| MPO | Sense, 5′-ATGCAGTGGGGACAGTTTCTG-3′ | 55 | 30 |
| Antisense, 5′-GTCGTTGTAGGATCGGTACTG-3′ | |||
| CEBP-α | Sense, 5′-GAACAGCAACGAGTACCGGGTA-3′ | 55 | 35 |
| Antisense, 5′-GCCATGGCCTTGACCAAGGAG-3′ | |||
| CEBP-ϵ | Sense, 5′-AGCCCCCGACACCCTTGATGA-3′ | 68 | 31 |
| Antisense, 5′-GTCCCCTTCTCAAGGCACCCT-3′ | |||
| Egr-1 | Sense, 5′-CAACTACCCCAAACTGGAGGAG-3′ | 56 | 32 |
| Antisense, 5′-GAGGATTGGTCATGCTCACGAG-3′ | |||
| SR | Sense, 5′-GTACTAATACCTGTTGTTGGA-3′ | 55 | 35 |
| Antisense, 5′-CGTGCGCTTGTTCTTCTTTCA-3′ | |||
| β-Actin | Sense, 5′-TGGCACCACACCTTCTACAA-3′ | 60 | 25 |
| Antisense, 5′-AGGAGCCAGAGCAGTAATCT-3′ |
Genes . | Primers . | Annealing temperature, °C . | Cycles . |
|---|---|---|---|
| BCR/ABL | Sense, 5′-AGCATTCCGCTGACCATCA-3′ | 62 | 30 |
| Antisense, 5′-AGCGGCTTCACTCAGACCC-3′ | |||
| ICSBP | Sense, 5′-AGAGGGAGACAAAGCTGAACC-3′ | 59 | 30 |
| Antisense, 5′-TGAATGGTGTGTGTCATAGGC-3′ | |||
| G-CSFR | Sense, 5′-CTCAAACCTATCCTGCCTCATG-3′ | 60 | 35 |
| Antisense, 5′-TCCAGGCAGAGATGAGCGAATG-3′ | |||
| c-fms | Sense, 5′-GCGATGTGTGAGCAATGGCAGT-3′ | 62 | 35 |
| Antisense, 5′-AGACCGTTTTGCGTAAGACCTG-3′ | |||
| GM-CSFR-α | Sense, 5′-GAGGTCCACAGGTCAAGGTG-3′ | 60 | 35 |
| Antisense, 5′-GATTGACAGTGGCAGGCTTC-3′ | |||
| GM-CSFR-β | Sense, 5′-TTTCCATCACAAACGAAGACT-3′ | 60 | 35 |
| Antisense, 5′-AATGAATGAGTAAGCCATCTT-3′ | |||
| PU.1 | Sense, 5′-TCTGATGGAGAAGCTGATGG-3′ | 55 | 31 |
| Antisense, 5′-CTTGACTTTCTTCACCTCGC-3′ | |||
| MPO | Sense, 5′-ATGCAGTGGGGACAGTTTCTG-3′ | 55 | 30 |
| Antisense, 5′-GTCGTTGTAGGATCGGTACTG-3′ | |||
| CEBP-α | Sense, 5′-GAACAGCAACGAGTACCGGGTA-3′ | 55 | 35 |
| Antisense, 5′-GCCATGGCCTTGACCAAGGAG-3′ | |||
| CEBP-ϵ | Sense, 5′-AGCCCCCGACACCCTTGATGA-3′ | 68 | 31 |
| Antisense, 5′-GTCCCCTTCTCAAGGCACCCT-3′ | |||
| Egr-1 | Sense, 5′-CAACTACCCCAAACTGGAGGAG-3′ | 56 | 32 |
| Antisense, 5′-GAGGATTGGTCATGCTCACGAG-3′ | |||
| SR | Sense, 5′-GTACTAATACCTGTTGTTGGA-3′ | 55 | 35 |
| Antisense, 5′-CGTGCGCTTGTTCTTCTTTCA-3′ | |||
| β-Actin | Sense, 5′-TGGCACCACACCTTCTACAA-3′ | 60 | 25 |
| Antisense, 5′-AGGAGCCAGAGCAGTAATCT-3′ |