Table 1. KIR immunophenotype of CD56 or... | American Society of Hematology

Table 1.

KIR immunophenotype of CD56⁺ or CD16⁺ NK cells in patients with NK-LDGL

	CD158a, 2DS1/2DL1, %	CD158b, 2DS2/2DL2/2DL3, %	NKB1, 3DL1, %	KARp50, 2DS4, %	KIR2DL4, %	CD94			NKG2A			NKG2D			% Total CD56⁺ or CD16⁺ NK cells†
Antibodies	CD158a, 2DS1/2DL1, %	CD158b, 2DS2/2DL2/2DL3, %	NKB1, 3DL1, %	KARp50, 2DS4, %	KIR2DL4, %						%	MFI	%	MFI	% Total CD56⁺ or CD16⁺ NK cells†	%	MFI
NKLDGL1	0	0.6	1.3	1.3	ND	38	392	0	0	66	200	66
NKLDGL2	0.4	0.8	0	4	21^*	40	505	42	668	86	204	42
NKLDGL3	0.2	3	0	0	1.5	45	1225	46	733	44	35	48
NKLDGL4	0.1	0.1	0.1	0.2	ND	74	400	75	32	ND	ND	74
NKDLGL5	76^*	4	0	0.8	19	78	519	78	234	68	135	78
NKLDGL6	0.7	80^*	0.3	4	ND	83	1211	83	161	74	17	83
NKLDGL7	0.5	0.8	1.6	0.6	ND	75	304	73	86	ND	ND	75
NKLDGL8	0.6	0.7	92^*	0.2	4	94	757	3	96	77	20	94
NKLDGL9	0.1	3.6	36^*	0	ND	44	198	42	185	ND	ND	45
NKLDGL10	3	0.2	0.2	41^*	ND	55	462	58	176	ND	ND	58
NKLDGL11	45	51	26	0	ND	35	124	2	170	ND	ND	60
NKLDGL12	0.8	2.5	19	0.8	81^*	83	403	83	259	81	249	84
NKLDGL13	8.7	12	15.6	0	ND	19	140	6	68	1.6	22	79
Average	—	—	—	—	—	59 ± 23	511 ± 356	45 ± 33	221 ± 226	62 ± 28	110 ± 97	68 ± 16
Normal 1	1.8	2.4	0.1	0.5	1.4	5.6	200	4	77	1.6	28	8
Normal 2	1.4	2.8	0.6	0.4	2.2	12	168	5.8	88	1.5	66	17
Normal 3	3.5	4	0.8	0.5	3	15	122	9.2	70	0.3	24	26
Normal 4	1.2	2.2	1.4	4.8	3.7	10	170	3.7	51	3	25	19
Average	—	—	—	—	—	11 ± 4	165 ± 32	6 ± 3	71 ± 16	1.6 ± 1	36 ± 20	18 ± 7

	CD158a, 2DS1/2DL1, %	CD158b, 2DS2/2DL2/2DL3, %	NKB1, 3DL1, %	KARp50, 2DS4, %	KIR2DL4, %	CD94			NKG2A			NKG2D			% Total CD56⁺ or CD16⁺ NK cells†
Antibodies	CD158a, 2DS1/2DL1, %	CD158b, 2DS2/2DL2/2DL3, %	NKB1, 3DL1, %	KARp50, 2DS4, %	KIR2DL4, %						%	MFI	%	MFI	% Total CD56⁺ or CD16⁺ NK cells†	%	MFI
NKLDGL1	0	0.6	1.3	1.3	ND	38	392	0	0	66	200	66
NKLDGL2	0.4	0.8	0	4	21^*	40	505	42	668	86	204	42
NKLDGL3	0.2	3	0	0	1.5	45	1225	46	733	44	35	48
NKLDGL4	0.1	0.1	0.1	0.2	ND	74	400	75	32	ND	ND	74
NKDLGL5	76^*	4	0	0.8	19	78	519	78	234	68	135	78
NKLDGL6	0.7	80^*	0.3	4	ND	83	1211	83	161	74	17	83
NKLDGL7	0.5	0.8	1.6	0.6	ND	75	304	73	86	ND	ND	75
NKLDGL8	0.6	0.7	92^*	0.2	4	94	757	3	96	77	20	94
NKLDGL9	0.1	3.6	36^*	0	ND	44	198	42	185	ND	ND	45
NKLDGL10	3	0.2	0.2	41^*	ND	55	462	58	176	ND	ND	58
NKLDGL11	45	51	26	0	ND	35	124	2	170	ND	ND	60
NKLDGL12	0.8	2.5	19	0.8	81^*	83	403	83	259	81	249	84
NKLDGL13	8.7	12	15.6	0	ND	19	140	6	68	1.6	22	79
Average	—	—	—	—	—	59 ± 23	511 ± 356	45 ± 33	221 ± 226	62 ± 28	110 ± 97	68 ± 16
Normal 1	1.8	2.4	0.1	0.5	1.4	5.6	200	4	77	1.6	28	8
Normal 2	1.4	2.8	0.6	0.4	2.2	12	168	5.8	88	1.5	66	17
Normal 3	3.5	4	0.8	0.5	3	15	122	9.2	70	0.3	24	26
Normal 4	1.2	2.2	1.4	4.8	3.7	10	170	3.7	51	3	25	19
Average	—	—	—	—	—	11 ± 4	165 ± 32	6 ± 3	71 ± 16	1.6 ± 1	36 ± 20	18 ± 7

KIR expression was determined by flow cytometry on PBMCs. Values indicate the % of CD16⁺ or CD56⁺ cells that coexpress KIR. Values shown as % positive = numbers obtained from the top right-hand quadrant of 2-color dot plot flow cytometry graphs.

More than 50% of the NK population has homogenous reactivity to a single KIR antibody.

†

The number in the top right added to the number in the top left quadrants of dot plot flow cytometry = total number of NK cells.

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