HLA antigen expression on bone marrow AML and CD33+ healthy donor progenitors
. | MFI of AML cells (SD) . | MFI of CD33+ normal progenitors (SD) . | P . |
|---|---|---|---|
| HLA-A, -B, -C | 53(32) | 15(11) | .0032 |
| HLA-A | 32(22) | 19(10) | NS |
| HLA-B | 41(26) | 26(15) | NS |
| β2m | 62(52) | 21(19) | .0119 |
| HLA-DR, -DQ, -DP | 28(31) | 7(2) | .0329 |
| HLA-DR | 33(31) | 6(2) | .0013 |
. | MFI of AML cells (SD) . | MFI of CD33+ normal progenitors (SD) . | P . |
|---|---|---|---|
| HLA-A, -B, -C | 53(32) | 15(11) | .0032 |
| HLA-A | 32(22) | 19(10) | NS |
| HLA-B | 41(26) | 26(15) | NS |
| β2m | 62(52) | 21(19) | .0119 |
| HLA-DR, -DQ, -DP | 28(31) | 7(2) | .0329 |
| HLA-DR | 33(31) | 6(2) | .0013 |
AML cells at diagnosis and HLA-matched bone marrow–derived CD33+ progenitors from healthy donors were stained with anti-HLA mAbs and analyzed by flow cytometry. All cells tested were untouched CD33+ cells obtained from immunomagnetic isolation procedures. Results obtained with AML cells and progenitors from healthy donors were compared using the nonparametric Mann-Whitney U test. Populations are as follows: MFI of AML cells, n = 20; and MFI of CD33+ normal progenitors, n = 10. NS indicates not significant.