HLA antigen expression on CLL-B and ALL-B cells
. | MFI of normal B cells (SD) . | MFI of CLL-B cells (SD) . | P . | MFI of ALL-B cells (SD) . | P . |
|---|---|---|---|---|---|
| HLA-A, -B, -C | 87 (33) | 55 (39) | .0067 | 32 (24) | .0012 |
| HLA-A | 28 (8) | 22 (16) | NS | 16 (8) | .0002 |
| HLA-B | 33 (14) | 32 (14) | NS | 16 (10) | .005 |
| β2m | 69 (32) | 52 (37) | NS | 24 (15) | NS |
| HLA-DR, -DQ, -DP | 30 (7) | 17 (13) | .0134 | 22 (23) | NS |
| HLA-DR | 29 (2) | 19 (15) | .0452 | 26 (20) | NS |
. | MFI of normal B cells (SD) . | MFI of CLL-B cells (SD) . | P . | MFI of ALL-B cells (SD) . | P . |
|---|---|---|---|---|---|
| HLA-A, -B, -C | 87 (33) | 55 (39) | .0067 | 32 (24) | .0012 |
| HLA-A | 28 (8) | 22 (16) | NS | 16 (8) | .0002 |
| HLA-B | 33 (14) | 32 (14) | NS | 16 (10) | .005 |
| β2m | 69 (32) | 52 (37) | NS | 24 (15) | NS |
| HLA-DR, -DQ, -DP | 30 (7) | 17 (13) | .0134 | 22 (23) | NS |
| HLA-DR | 29 (2) | 19 (15) | .0452 | 26 (20) | NS |
CLL-B and ALL-B cells at diagnosis and peripheral blood B cells isolated from healthy donors were stained with anti-HLA mAbs and analyzed by flow cytometry. All cells tested were untouched fractions derived from immunomagnetic isolation procedures. Results are expressed as mean fluorescence intensity (MFI) values and standard deviation (SD). Results obtained with leukemic and normal B cells were compared using the nonparametric Mann-Whitney U test. Populations are as follows: MFI of normal B cells, n = 10; MFI of CLL-B cells, n = 17; and MFI of ALL-B cells, n = 9. NS indicates not significant.