Reactivity of HLA-B-specific humAbs with a panel of HLA-typed lymphocytes and cell lines
mAb . | Isotype . | Specificity . | PBLs . | Cell lines . | Flow cytometry . |
|---|---|---|---|---|---|
| 13E12 | IgM, κ | B12 | 7* | 0* | +++ |
| GK31F12 | IgM, κ | B13 | 4 | 0 | +++ |
| HA2C10B12 | IgM, κ | B60 (40) | 7 | 2 | +++ |
| VTM3A1 | IgG1, κ | B7 | 7 | 4 | +++ |
| VTM4D9 | IgG1, κ | B7 | 7 | 4 | ++ |
| DMS4G2 | IgG1, λ | B62 (15) | 2 | 2 | ++/+++ |
| DMS2G2 | IgG1, λ | B35 | 7 | 2 | +++ |
| KAL3D5 | IgG1, λ | B51 (5) | 5 | 2 | +++ |
| HDG8D9 | IgG1, λ | B51 (5) | 5 | 2 | +++ |
| GR5B3 | IgM, λ | B62 (15) | 2 | 2 | ++ |
| BVK5B10 | IgM, κ | B8 | 7 | 1 | +++ |
| AE9D9 | IgM, λ | B8 | 7 | 1 | + |
| AE9D9 | IgM, λ | B14 | 3 | 0 | 0 |
| OK6H10 | IgM, κ | B15 | 1 | 2 | +++ |
| OK6H10 | IgM, κ | B35 | 7 | 2 | +++ |
| SN230G6 | IgG1, λ | B17 | 3 | 0 | +++ |
| KG30A7 | IgM, λ | B12 | 7 | 1 | ++ |
| KG30A7 | IgM, λ | B14 | 4 | 0 | +++ |
| GVK2F8 | IgM, λ | B18 | 4 | 1 | +++ |
| GVK2F8 | IgM, λ | B39 (16) | 3 | 0 | +++ |
| HDG2G7 | IgG1, κ | B57 (17) | 2 | 0 | +++ |
| OK6H10 | IgM, κ | B35 | 7 | 2 | +++ |
| OK6H10 | IgM, κ | B15 | 1 | 2 | +++ |
| OK6H10 | IgM, κ | B15 | 1 | 2 | +++ |
| OK6H10 | IgM, κ | B35 | 7 | 2 | +++ |
| FVS4G4 | IgM, κ | B35 | 7 | 2 | +++ |
| FVS4G4 | IgM, κ | B17 | 6 | 0 | + |
| FVS4G4 | IgM, κ | B62 (15) | 2 | 2 | +++ |
| FVS4G4 | IgM, κ | B51 (5) | 5 | 2 | +++ |
| FVS4G4 | IgM, κ | B14 | 5 | 0 | +++ |
| FVS4G4 | IgM, κ | B18 | 4 | 1 | ++/+++ |
| FVS4G4 | IgM, κ | B38 (16) | 3 | 1 | +++ |
mAb . | Isotype . | Specificity . | PBLs . | Cell lines . | Flow cytometry . |
|---|---|---|---|---|---|
| 13E12 | IgM, κ | B12 | 7* | 0* | +++ |
| GK31F12 | IgM, κ | B13 | 4 | 0 | +++ |
| HA2C10B12 | IgM, κ | B60 (40) | 7 | 2 | +++ |
| VTM3A1 | IgG1, κ | B7 | 7 | 4 | +++ |
| VTM4D9 | IgG1, κ | B7 | 7 | 4 | ++ |
| DMS4G2 | IgG1, λ | B62 (15) | 2 | 2 | ++/+++ |
| DMS2G2 | IgG1, λ | B35 | 7 | 2 | +++ |
| KAL3D5 | IgG1, λ | B51 (5) | 5 | 2 | +++ |
| HDG8D9 | IgG1, λ | B51 (5) | 5 | 2 | +++ |
| GR5B3 | IgM, λ | B62 (15) | 2 | 2 | ++ |
| BVK5B10 | IgM, κ | B8 | 7 | 1 | +++ |
| AE9D9 | IgM, λ | B8 | 7 | 1 | + |
| AE9D9 | IgM, λ | B14 | 3 | 0 | 0 |
| OK6H10 | IgM, κ | B15 | 1 | 2 | +++ |
| OK6H10 | IgM, κ | B35 | 7 | 2 | +++ |
| SN230G6 | IgG1, λ | B17 | 3 | 0 | +++ |
| KG30A7 | IgM, λ | B12 | 7 | 1 | ++ |
| KG30A7 | IgM, λ | B14 | 4 | 0 | +++ |
| GVK2F8 | IgM, λ | B18 | 4 | 1 | +++ |
| GVK2F8 | IgM, λ | B39 (16) | 3 | 0 | +++ |
| HDG2G7 | IgG1, κ | B57 (17) | 2 | 0 | +++ |
| OK6H10 | IgM, κ | B35 | 7 | 2 | +++ |
| OK6H10 | IgM, κ | B15 | 1 | 2 | +++ |
| OK6H10 | IgM, κ | B15 | 1 | 2 | +++ |
| OK6H10 | IgM, κ | B35 | 7 | 2 | +++ |
| FVS4G4 | IgM, κ | B35 | 7 | 2 | +++ |
| FVS4G4 | IgM, κ | B17 | 6 | 0 | + |
| FVS4G4 | IgM, κ | B62 (15) | 2 | 2 | +++ |
| FVS4G4 | IgM, κ | B51 (5) | 5 | 2 | +++ |
| FVS4G4 | IgM, κ | B14 | 5 | 0 | +++ |
| FVS4G4 | IgM, κ | B18 | 4 | 1 | ++/+++ |
| FVS4G4 | IgM, κ | B38 (16) | 3 | 1 | +++ |
HLA-B allele–specific mAbs were tested with HLA class I–typed peripheral blood lymphocytes (PBLs) and cell lines in indirect immunofluorescence (IIF). Two isotype-matched controls were included in every experiment. All mAbs stained all cell samples expressing the corresponding HLA class I allospecificity. Reactivity is expressed as 1 log (+), 2 logs (++), or 3 logs (+++) stronger than the negative controls used; 0 indicates no reaction. Numbers in parentheses indicate broad specificity.
Number of samples tested.