Relative repopulating abilities of SCL-heterozygous and SCL-deleted bone marrow
Experiment (donor RUs/competitor RUs, × 105) . | SCL-heterozygous . | SCL-deleted . | Fold reduction . |
|---|---|---|---|
| 1 (20/5) | |||
| Myeloid lineage | 67 (10) | 11 (0.62) | 16.5 |
| B-cell lineage | 85 (28) | 34 (2.6) | 10.8 |
| T-cell lineage | 90 (45) | 29 (2.0) | 22.5 |
| 2 (20/5) | |||
| Myeloid lineage | 69 (11) | 22 (1.4) | 7.9 |
| B-cell lineage | 82 (22) | 53 (5.6) | 4.0 |
| T-cell lineage | ND | ND | ND |
| 3 (20/2) | |||
| Myeloid lineage | 87 (13) | 34 (1.0) | 13 |
| B-cell lineage | 86 (12) | 56 (2.5) | 4.8 |
| T-cell lineage | 87 (13) | 41 (1.4) | 9.3 |
Experiment (donor RUs/competitor RUs, × 105) . | SCL-heterozygous . | SCL-deleted . | Fold reduction . |
|---|---|---|---|
| 1 (20/5) | |||
| Myeloid lineage | 67 (10) | 11 (0.62) | 16.5 |
| B-cell lineage | 85 (28) | 34 (2.6) | 10.8 |
| T-cell lineage | 90 (45) | 29 (2.0) | 22.5 |
| 2 (20/5) | |||
| Myeloid lineage | 69 (11) | 22 (1.4) | 7.9 |
| B-cell lineage | 82 (22) | 53 (5.6) | 4.0 |
| T-cell lineage | ND | ND | ND |
| 3 (20/2) | |||
| Myeloid lineage | 87 (13) | 34 (1.0) | 13 |
| B-cell lineage | 86 (12) | 56 (2.5) | 4.8 |
| T-cell lineage | 87 (13) | 41 (1.4) | 9.3 |
Repopulating units (RUs) are defined as the repopulating ability of 1 × 105 fresh marrow cells from the SCL-wild-type competitor. The mean percentage of donor-derived cells for each lineage at 16 weeks are given, each calculated from a group of 3 to 5 recipients. The method of calculation for the RUs given in parentheses is described in “Materials and methods.” The fold reduction was calculated by dividing the RUs of SCL-heterozygous donor cells by the RUs of SCL-deleted donor cells.
ND indicates not done.