Table 2.

Effect of enforced expression of TAL1 and ΔbTAL1 on the LTC-IC compartment


Transgene

Percent of CD34+ cells*

No. of cells × 106

Total CFCs/well

Total CFCs × 103
EGFP  60   3.3   416   306  
TAL1  91   5.4   823   739 (×2.4) 
ΔbTAL1  91   3.7   482   307  
EGFP§  53   3.1   124   43  
TAL1§  67   4.4   321   132 (×3) 
ΔbTAL1§
 
88
 
5.5
 
80
 
45
 

Transgene

Percent of CD34+ cells*

No. of cells × 106

Total CFCs/well

Total CFCs × 103
EGFP  60   3.3   416   306  
TAL1  91   5.4   823   739 (×2.4) 
ΔbTAL1  91   3.7   482   307  
EGFP§  53   3.1   124   43  
TAL1§  67   4.4   321   132 (×3) 
ΔbTAL1§
 
88
 
5.5
 
80
 
45
 
*

Percent evaluated by flow cytometry.

Calculated based on the FACS data and the proliferation rate in the culture.

Experiment 1, day 2; 3000 cells/well. See notes to Table 1.

§

Experiment 2, day 3; 5000 cells/well. See notes to Table 1.

Values in parentheses indicate fold increase compared with EGFP.

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