Effect of enforced TAL1 and ΔbTAL1 expression on human CFCs
Cells and transgenes . | Percent of cells* . | No. of cells × 106† . | CFCs/1000 cells . | Total CFCs‡× 103 . | Percent of progenitors (absolute no., × 103) . | . | . | ||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | CFU-Es . | BFU-Es . | CFU-GMs . | ||
| CD34+CD36−§ | |||||||||
| EGFP | 40 | 2.2 | 174 | 383 | 1 | 51 | 48 | ||
| TAL1 | 46 | 2.7 | 176 | 475 | 2 | 50 | 48 | ||
| ΔbTAL1 | 49 | 2 | 177 | 354 | 3 | 48 | 49 | ||
| CD34+CD36+§ | |||||||||
| EGFP | 20 | 1.1 | 162 | 178 | 2.5 (4.5) | 92 (163) | 5.5 | ||
| TAL1 | 45 | 2.7 | 304 | 821 | 14.5 (126) | 81.5 (669) | 4 | ||
| ΔbTAL1 | 42 | 1.7 | 194 | 330 | 30.5 (101) | 65.5 (216) | 4 | ||
| CD34+CD36−∥ | |||||||||
| EGFP | 29 | 1.7 | 170 | 292 | 0 | 42 | 58 | ||
| TAL1 | 31 | 2 | 150 | 307 | 1 | 45 | 55 | ||
| ΔbTAL1 | 45 | 2.8 | 130 | 363 | 0 | 27 | 74 | ||
| CD34+CD36+∥ | |||||||||
| EGFP | 24 | 1.4 | 302 | 423 | 3 (13) | 91 (384) | 5 | ||
| TAL1 | 36 | 2.4 | 321 | 761 | 20 (152) | 75 (570) | 5 | ||
| ΔbTAL1 | 43 | 2.7 | 325 | 865 | 18.5 (160) | 78 (674) | 3 | ||
Cells and transgenes . | Percent of cells* . | No. of cells × 106† . | CFCs/1000 cells . | Total CFCs‡× 103 . | Percent of progenitors (absolute no., × 103) . | . | . | ||
|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | CFU-Es . | BFU-Es . | CFU-GMs . | ||
| CD34+CD36−§ | |||||||||
| EGFP | 40 | 2.2 | 174 | 383 | 1 | 51 | 48 | ||
| TAL1 | 46 | 2.7 | 176 | 475 | 2 | 50 | 48 | ||
| ΔbTAL1 | 49 | 2 | 177 | 354 | 3 | 48 | 49 | ||
| CD34+CD36+§ | |||||||||
| EGFP | 20 | 1.1 | 162 | 178 | 2.5 (4.5) | 92 (163) | 5.5 | ||
| TAL1 | 45 | 2.7 | 304 | 821 | 14.5 (126) | 81.5 (669) | 4 | ||
| ΔbTAL1 | 42 | 1.7 | 194 | 330 | 30.5 (101) | 65.5 (216) | 4 | ||
| CD34+CD36−∥ | |||||||||
| EGFP | 29 | 1.7 | 170 | 292 | 0 | 42 | 58 | ||
| TAL1 | 31 | 2 | 150 | 307 | 1 | 45 | 55 | ||
| ΔbTAL1 | 45 | 2.8 | 130 | 363 | 0 | 27 | 74 | ||
| CD34+CD36+∥ | |||||||||
| EGFP | 24 | 1.4 | 302 | 423 | 3 (13) | 91 (384) | 5 | ||
| TAL1 | 36 | 2.4 | 321 | 761 | 20 (152) | 75 (570) | 5 | ||
| ΔbTAL1 | 43 | 2.7 | 325 | 865 | 18.5 (160) | 78 (674) | 3 | ||
Percent evaluated by flow cytometry.
Calculated based on the FACS data and the proliferation rate in the culture.
Number of CFCs generated during the erythroid culture.
Experiment 1 cells were sorted after 2 days of culture in presence of SCF, IL-3, and IL-6.
Experiment 2 cells were sorted after 3 days of culture in presence of SCF, IL-3, and IL-6.