Confirmation of microarray data by real-time RT-PCR: TNF-α—mediated differential gene expression in HUVECs
Genes . | Accession no. . | Fold-change in wild-type HUVECs after exposure to TNF-α, analyzed by real-time RT-PCR . | IKK2/NF-kB—dependent regulation, analyzed by real-time RT-PCR . | p38 MAP kinase—dependent regulation, analyzed by real-time RT-PCR . | Confirmation of complete microarray data set by real-time RT-PCR . |
|---|---|---|---|---|---|
| Bone morphogenetic protein 2* | AA583044 | 6.0 | Yes | Yes | Yes |
| VCAM-1 | NM_001078.1 | 132.5 | Yes | Yes | Yes |
| ICAM-1 | NM_000201.1 | 65.3 | Yes | No | Yes |
| CD137† | NM_001561.2 | 32.9 | Yes | No | IKK2/NF-kB dependence not detected by microarray |
| Cyclo-oxygenase 2‡ | NM_000963.1 | 4.1 | ND | Yes | Yes |
| TRAIL§ | NM_003810.1 | 4.9 | Yes | Yes | Yes |
| c-IAP-2∥ | U37546.1 | 84.5 | Yes | No | Yes |
| Clone IMAGE:4711494, mRNA | BF575213 | 16.0 | Yes | No | Yes |
| Coxsackie virus and adenovirus receptor¶ | NM_001338.1 | −30.9 | Yes | No | Yes |
Genes . | Accession no. . | Fold-change in wild-type HUVECs after exposure to TNF-α, analyzed by real-time RT-PCR . | IKK2/NF-kB—dependent regulation, analyzed by real-time RT-PCR . | p38 MAP kinase—dependent regulation, analyzed by real-time RT-PCR . | Confirmation of complete microarray data set by real-time RT-PCR . |
|---|---|---|---|---|---|
| Bone morphogenetic protein 2* | AA583044 | 6.0 | Yes | Yes | Yes |
| VCAM-1 | NM_001078.1 | 132.5 | Yes | Yes | Yes |
| ICAM-1 | NM_000201.1 | 65.3 | Yes | No | Yes |
| CD137† | NM_001561.2 | 32.9 | Yes | No | IKK2/NF-kB dependence not detected by microarray |
| Cyclo-oxygenase 2‡ | NM_000963.1 | 4.1 | ND | Yes | Yes |
| TRAIL§ | NM_003810.1 | 4.9 | Yes | Yes | Yes |
| c-IAP-2∥ | U37546.1 | 84.5 | Yes | No | Yes |
| Clone IMAGE:4711494, mRNA | BF575213 | 16.0 | Yes | No | Yes |
| Coxsackie virus and adenovirus receptor¶ | NM_001338.1 | −30.9 | Yes | No | Yes |
ND indicates not detectable, since strict statistical criteria do not appraise the gene transcript in empty expression vector—infected HUVECs as TNF-α induced.
BMP2.
TNF receptor superfamily member 9.
Cox2, prostaglandin-endoperoxidase synthase 2.
Tumor necrosis factor (ligand) superfamily member 10.
Baculoviral IAP repeat—containing 3.
CAR.