TNF-α-mediated up-regulation of selected IKK2/NF-κB- and p38 MAP kinase-dependent genes in endothelial cells (HUVECs)
Genes up-regulated by TNF-α . | Accession no. . | Fold-change or grade of expression over background in wild-type HUVECsa . | P . | Induction dependent on IKK2/NF-κB, % . | Induction dependent on p38 MAP kinase, % . |
|---|---|---|---|---|---|
| Chemokines/cytokines | |||||
| IL-8 | AF043337.1 | 17.3 | < .001 | 100.0 | 53.1 |
| Bone morphogenetic protein 2 | AA583044 | 4.8 | < .001 | 77.4 | 50.9 |
| Inhibin beta Ab | M13436.1 | 3.1 | < .001 | 100.0 | 44.7 |
| CXCL6c | NM_002993.1 | 2.7 | < .001 | 100.0 | No |
| Fraktalkined | NM_002996.1 | +++ | < .05 | 100 | No |
| Cell-surface receptors | |||||
| VCAM-1 | NM_001078.1 | 38.1 | < .001 | 100.0 | 52.7 |
| E-selectin | NM_000450.1 | 35.7 | < .001 | 100.0 | No |
| ICAM-1 | NM_000201.1 | 22.1 | < .001 | 100.0 | No |
| CD137 antigene | NM_001561.2 | 3.0 | < .001 | ND | No |
| CD69 antigen | L07555.1 | ++ | < .05 | 100.0 | No |
| Coagulation | |||||
| Coagulation factor IIIf | NM_001993.2 | ++ | < .05 | ND | 100.0 |
| Inflammation/immune response | |||||
| Cyclo-oxygenase 2g | NM_000963.1 | 2.9 | < .005 | ND | 100.0 |
| Signaling/transcription | |||||
| Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alphah | AI078167 | 7.4 | < .001 | 100.0 | No |
| Jagged 1 | U77914.1 | 5.0 | < .001 | 100.0 | No |
| Nef-associated factor 1i | NM_006058.1 | 4.7 | < .001 | 100.0 | No |
| Ephrin-A1j | NM_004428.1 | 4.5 | < .001 | 78.9 | No |
| Jun B proto-oncogene | NM_002229.1 | 3.2 | < .001 | No | No |
| Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2k | BC002844.1 | 3.0 | < .05492 | 100.0 | No |
| Nuclear receptor interacting protein 1l | A1824012 | 2.9 | < .001 | 100 | 44.0 |
| Apoptosis/cell proliferation | |||||
| TNF-α-induced protein 3m | NM_006290.1 | 13.0 | < .001 | 100.0 | No |
| TRAILn | NM_003810.1 | 4.0 | < .005 | ND | 46.4 |
| c-IAP-2o | U37546.1 | ++ | < .05 | 100.0 | No |
| Metabolism | |||||
| Guanylate binding protein 1, interferon-inducible, 67 kDa | NM_002053.1 | 3.6 | < .001 | 100.0 | 49.3 |
| Transporters/channels | |||||
| Transporter 1, ATP-binding cassette, subfamily B | NM_000593.2 | + | < .05 | 100.0 | No |
| Others | |||||
| HIV type 1 enhancer binding protein 2p | AL023584 | 4.0 | < .001 | 66.0 | No |
Genes up-regulated by TNF-α . | Accession no. . | Fold-change or grade of expression over background in wild-type HUVECsa . | P . | Induction dependent on IKK2/NF-κB, % . | Induction dependent on p38 MAP kinase, % . |
|---|---|---|---|---|---|
| Chemokines/cytokines | |||||
| IL-8 | AF043337.1 | 17.3 | < .001 | 100.0 | 53.1 |
| Bone morphogenetic protein 2 | AA583044 | 4.8 | < .001 | 77.4 | 50.9 |
| Inhibin beta Ab | M13436.1 | 3.1 | < .001 | 100.0 | 44.7 |
| CXCL6c | NM_002993.1 | 2.7 | < .001 | 100.0 | No |
| Fraktalkined | NM_002996.1 | +++ | < .05 | 100 | No |
| Cell-surface receptors | |||||
| VCAM-1 | NM_001078.1 | 38.1 | < .001 | 100.0 | 52.7 |
| E-selectin | NM_000450.1 | 35.7 | < .001 | 100.0 | No |
| ICAM-1 | NM_000201.1 | 22.1 | < .001 | 100.0 | No |
| CD137 antigene | NM_001561.2 | 3.0 | < .001 | ND | No |
| CD69 antigen | L07555.1 | ++ | < .05 | 100.0 | No |
| Coagulation | |||||
| Coagulation factor IIIf | NM_001993.2 | ++ | < .05 | ND | 100.0 |
| Inflammation/immune response | |||||
| Cyclo-oxygenase 2g | NM_000963.1 | 2.9 | < .005 | ND | 100.0 |
| Signaling/transcription | |||||
| Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alphah | AI078167 | 7.4 | < .001 | 100.0 | No |
| Jagged 1 | U77914.1 | 5.0 | < .001 | 100.0 | No |
| Nef-associated factor 1i | NM_006058.1 | 4.7 | < .001 | 100.0 | No |
| Ephrin-A1j | NM_004428.1 | 4.5 | < .001 | 78.9 | No |
| Jun B proto-oncogene | NM_002229.1 | 3.2 | < .001 | No | No |
| Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2k | BC002844.1 | 3.0 | < .05492 | 100.0 | No |
| Nuclear receptor interacting protein 1l | A1824012 | 2.9 | < .001 | 100 | 44.0 |
| Apoptosis/cell proliferation | |||||
| TNF-α-induced protein 3m | NM_006290.1 | 13.0 | < .001 | 100.0 | No |
| TRAILn | NM_003810.1 | 4.0 | < .005 | ND | 46.4 |
| c-IAP-2o | U37546.1 | ++ | < .05 | 100.0 | No |
| Metabolism | |||||
| Guanylate binding protein 1, interferon-inducible, 67 kDa | NM_002053.1 | 3.6 | < .001 | 100.0 | 49.3 |
| Transporters/channels | |||||
| Transporter 1, ATP-binding cassette, subfamily B | NM_000593.2 | + | < .05 | 100.0 | No |
| Others | |||||
| HIV type 1 enhancer binding protein 2p | AL023584 | 4.0 | < .001 | 66.0 | No |
Data shown in bold represent observations that have so far not been reported in literature.
ND indicates not detectable, since our strict statistical criteria do not appraise the respective gene transcripts in empty expression vector-infected HUVECs as TNF-α inducible; and +++ indicates an expression over background with an n-fold range of 25-50; ++, n-fold range of 10 to 25; and +, n-fold range of 5 to 10.
For details, see “Materials and methods.”
Activin beta-A chain.
GCP-2.
CX3CL1.
TNF receptor superfamily, member 9.
Thromboplastin, tissue factor, CD142.
Cox2, prostaglandin-endoperoxidase synthase 2.
IκBα.
Naf1.
TNF-α-induced protein 4.
p49/p100.
NRIP-1.
A20.
Tumor necrosis factor (ligand) superfamily; member 10.
Baculoviral IAP repeat-containing 3.
HIVEP2.