Gene expression pattern in response to injected truncated c-met mRNA
. | . | Gene expression in ventral-vegetable injections, % . | . | . | . | Gene expression in dorsal-vegetable injections, % . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | Decreased or absent . | . | . | . | Decreased or absent . | . | ||||||
| RNA . | In situ . | n . | Normal . | Posterior VBI . | Throughout VBI . | n . | Normal . | Anterior VBI . | Throughout VBI . | ||||||
| tc-met | α-globin | 42 | 33 | 67 | 0 | 46 | 9 | 71 | 20 | ||||||
| LacZ | α-globin | 36 | 86 | 14 | 0 | 28 | 74 | 15 | 11 | ||||||
| tc-met | GATA-1 | 34 | 39 | 61 | 0 | 39 | 23 | 67 | 10 | ||||||
| LacZ | GATA-1 | 37 | 78 | 19 | 3 | 31 | 81 | 6 | 13 | ||||||
| tc-met | SCL | 39 | 49 | 51 | 0 | 41 | 20 | 78 | 2 | ||||||
| LacZ | SCL | 25 | 92 | 8 | 0 | 32 | 91 | 9 | 0 | ||||||
| tc-met | GATA-2 | 33 | 100 | 0 | 0 | 29 | 82 | 14 | 4 | ||||||
| LacZ | GATA-2 | 31 | 97 | 0 | 3 | 40 | 82 | 5 | 13 | ||||||
. | . | Gene expression in ventral-vegetable injections, % . | . | . | . | Gene expression in dorsal-vegetable injections, % . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | Decreased or absent . | . | . | . | Decreased or absent . | . | ||||||
| RNA . | In situ . | n . | Normal . | Posterior VBI . | Throughout VBI . | n . | Normal . | Anterior VBI . | Throughout VBI . | ||||||
| tc-met | α-globin | 42 | 33 | 67 | 0 | 46 | 9 | 71 | 20 | ||||||
| LacZ | α-globin | 36 | 86 | 14 | 0 | 28 | 74 | 15 | 11 | ||||||
| tc-met | GATA-1 | 34 | 39 | 61 | 0 | 39 | 23 | 67 | 10 | ||||||
| LacZ | GATA-1 | 37 | 78 | 19 | 3 | 31 | 81 | 6 | 13 | ||||||
| tc-met | SCL | 39 | 49 | 51 | 0 | 41 | 20 | 78 | 2 | ||||||
| LacZ | SCL | 25 | 92 | 8 | 0 | 32 | 91 | 9 | 0 | ||||||
| tc-met | GATA-2 | 33 | 100 | 0 | 0 | 29 | 82 | 14 | 4 | ||||||
| LacZ | GATA-2 | 31 | 97 | 0 | 3 | 40 | 82 | 5 | 13 | ||||||
Summary of gene expression pattern obtained in injection experiments. Frequency of gene expression pattern is given as a percentage. n represents the number of embryos scored from independent experiments. Expressions of genes were analyzed by in situ hybridization at stage 33/34 (α-globin), stage 32/33 (GATA-1), and stage 30/31 (SCL and GATA-2). Embryos were scored for decreased or absent staining throughout the VBI or the posterior portion of the VBI in embryos injected with mRNAs to ventral-vegetable blastomeres, and throughout the VBI or the anterior portion of the VBI in embryos injected to dorsal-vegetable blastomeres.
tc-met indicates truncated c-met.