Uninfected erythrocytes inhibit phagocytosis of iRBCs by peripheral blood monocytes
. | Percentage of monocytes containing phagocytosed iRBCs or latex beads . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | Adherent cells . | . | Detached monocytes . | . | |||
| Incubation time and donor . | Static . | Shaken . | Static . | Shaken . | |||
| 2 h incubation | |||||||
| Donor no. 9 | |||||||
| iRBCs without uRBCs | 14 | 23 | — | — | |||
| iRBCs with uRBCs | 1 | 0 | 4 | — | |||
| Beads without uRBCs | 39.5 | 15 | — | — | |||
| Beads with uRBCs | 0.5 | 2 | 36.7 | — | |||
| Donor no. 15 | |||||||
| iRBCs without uRBCs | 11 | 6 | — | — | |||
| iRBCs with uRBCs | 0 | 1 | 7 | 12.8 | |||
| Beads without uRBCs | 27.5 | 43 | — | — | |||
| Beads with uRBCs | 0 | 11 | 66 | 50 | |||
| Donor no. 16 | |||||||
| iRBCs without uRBCs | 23 | 6 | — | — | |||
| iRBCs with uRBCs | 0.5 | 5 | 4 | 5 | |||
| Beads without uRBCs | 66 | 44 | — | — | |||
| Beads with uRBCs | 1.5 | 13 | 36 | 34 | |||
| 18 h incubation | |||||||
| Donor no. 6 | |||||||
| iRBCs without uRBCs | 41 | 44.5 | — | — | |||
| iRBCs with uRBCs | 2 | 5 | 7 | NP | |||
| Beads without uRBCs | 57 | 20.5 | — | — | |||
| Beads with uRBCs | 1 | 10.5 | 80 | NP | |||
| Donor no. 17 | |||||||
| iRBCs without uRBCs | 33 | 39.5 | NP | — | |||
| iRBCs with uRBCs | 1.67 | 8 | — | NP | |||
| Beads without uRBCs | 36 | 38.5 | NP | — | |||
| Beads with uRBCs | 2.9 | 8.5 | 52 | NP | |||
| Donor no. 5 | |||||||
| iRBCs without uRBCs | 32.5 | 31 | — | — | |||
| iRBCs with uRBCs | — | 6 | 12 | NP | |||
| Beads without uRBCs | 28.9 | 47 | — | — | |||
| Beads with uRBCs | 6.3 | 13 | 52 | 50 | |||
. | Percentage of monocytes containing phagocytosed iRBCs or latex beads . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| . | Adherent cells . | . | Detached monocytes . | . | |||
| Incubation time and donor . | Static . | Shaken . | Static . | Shaken . | |||
| 2 h incubation | |||||||
| Donor no. 9 | |||||||
| iRBCs without uRBCs | 14 | 23 | — | — | |||
| iRBCs with uRBCs | 1 | 0 | 4 | — | |||
| Beads without uRBCs | 39.5 | 15 | — | — | |||
| Beads with uRBCs | 0.5 | 2 | 36.7 | — | |||
| Donor no. 15 | |||||||
| iRBCs without uRBCs | 11 | 6 | — | — | |||
| iRBCs with uRBCs | 0 | 1 | 7 | 12.8 | |||
| Beads without uRBCs | 27.5 | 43 | — | — | |||
| Beads with uRBCs | 0 | 11 | 66 | 50 | |||
| Donor no. 16 | |||||||
| iRBCs without uRBCs | 23 | 6 | — | — | |||
| iRBCs with uRBCs | 0.5 | 5 | 4 | 5 | |||
| Beads without uRBCs | 66 | 44 | — | — | |||
| Beads with uRBCs | 1.5 | 13 | 36 | 34 | |||
| 18 h incubation | |||||||
| Donor no. 6 | |||||||
| iRBCs without uRBCs | 41 | 44.5 | — | — | |||
| iRBCs with uRBCs | 2 | 5 | 7 | NP | |||
| Beads without uRBCs | 57 | 20.5 | — | — | |||
| Beads with uRBCs | 1 | 10.5 | 80 | NP | |||
| Donor no. 17 | |||||||
| iRBCs without uRBCs | 33 | 39.5 | NP | — | |||
| iRBCs with uRBCs | 1.67 | 8 | — | NP | |||
| Beads without uRBCs | 36 | 38.5 | NP | — | |||
| Beads with uRBCs | 2.9 | 8.5 | 52 | NP | |||
| Donor no. 5 | |||||||
| iRBCs without uRBCs | 32.5 | 31 | — | — | |||
| iRBCs with uRBCs | — | 6 | 12 | NP | |||
| Beads without uRBCs | 28.9 | 47 | — | — | |||
| Beads with uRBCs | 6.3 | 13 | 52 | 50 | |||
Phagocytosis of iRBCs and latex beads by adherent and detached monocytes after 2 or 18 hours, in static or shaken cultures, was quantified by Giemsa staining of chamber-well slides or cytospin preparations, respectively. One hundred monocytes were counted for each duplicate slide, and the mean percentage of monocytes positive for phagocytosis of iRBCs or latex beads is given.
— indicates no monocytes seen on the slide; and NP, monocytes seen, but quantification of phagocytosis not possible.