Ectopic expression of Gas 1 inhibits apoptosis of 293 and endothelial cells
Cells and their transfected DNA . | No. of cells analyzed . | No. of GFP-positive cells . | No. of apoptotic GFP-positive cells . | No. of apoptotic GFP-negative cells . | Relative inhibition of apoptosis, % . |
|---|---|---|---|---|---|
| 293 | |||||
| GFP | 354 | 216 | 75 | 50 | 3 |
| GFP + Gas1 | 382 | 245 | 2 | 49 | 98 |
| VE-cadherin positive | |||||
| GFP | 425 | 382 | 48 | 6 | 13 |
| GFP + Gas1 | 453 | 410 | 13 | 6 | 77 |
| VE-cadherin null | |||||
| GFP | 410 | 372 | 145 | 16 | 7 |
| GFP + Gas1 | 434 | 391 | 12 | 17 | 92 |
Cells and their transfected DNA . | No. of cells analyzed . | No. of GFP-positive cells . | No. of apoptotic GFP-positive cells . | No. of apoptotic GFP-negative cells . | Relative inhibition of apoptosis, % . |
|---|---|---|---|---|---|
| 293 | |||||
| GFP | 354 | 216 | 75 | 50 | 3 |
| GFP + Gas1 | 382 | 245 | 2 | 49 | 98 |
| VE-cadherin positive | |||||
| GFP | 425 | 382 | 48 | 6 | 13 |
| GFP + Gas1 | 453 | 410 | 13 | 6 | 77 |
| VE-cadherin null | |||||
| GFP | 410 | 372 | 145 | 16 | 7 |
| GFP + Gas1 | 434 | 391 | 12 | 17 | 92 |
293, VE-cadherin–positive, and –null endothelial cells transfected either with GFP or GFP + Gas1 vector were cultured in absence of serum as described in “Materials and methods.” Apoptosis was measured by TUNEL assay. The values are means of triplicate from a typical experiment out of 3 performed. The relative inhibition of apoptosis was calculated by the following formula: percent = (% apoptotic GFP-negative cells - % apoptotic GFP-positive cells)/% apoptotic GFP-negative cells.