Analysis of novel genes identified in 6 huES cell lines
Gene name . | Chromosome location . | Predicted size . | Comments . |
|---|---|---|---|
| ZNF43 | 19p13.11 | 803 AA | Zinc finger proteins that share high sequence homology and likely function as transcriptional repressors; also known to be expressed in early development |
| ZNF257 | 19q13ÊÊ | 535 AA | |
| ZNF117 | 7q11.21 | 418 AA | |
| MGC27165 | Chr 14 | 5155 bases | Related to the fragilis group of proteins known to be germ cell–specific and critical for their maintenance |
| GSH1 | Chr 13 | 792 bases | GSH1 homeodomain protein containing a HOX domain |
| 264 AA | |||
| FLJ12581 (Nanog) | 12p13.31 | 6660 bases | ES cell–specific transcription factor critical for pluripotency |
| KIAA1573 | 1p13.3 | 3559 bases | Low homology to calcium channel, voltage-dependent, alpha 2/delta subunit 2 |
| Ribosomal 40A Laminin receptor | Chr 6 | — | Human DNA sequence from clone RP3-334F4 on chromosome 6 contains ESTs, STSs, and GSS: contains a LAMR1 (laminin receptor 1, ribosomal protein SA) pseudogene and an RPL10 (ribosomal protein L 10) pseudogene |
| C15orf15 | 15q21 | 15 719 bases | Chromosome 15 open reading frame 15 |
| C20orf129 | 20q11.22-q12 | 26 744 bases | Chromosome 20 open reading frame 129 |
| C20orf1 | 20q11.2 | 62 472 bases | Chromosome 20 open reading frame 1 |
| Numatrin | Chr 13 | — | Human DNA sequence from clone RP11-248N6 on chromosome 13 contains ESTs, STSs, and GSSs; contains 2 olfactory receptor pseudogenes, an NPM1 (nucleophosmin, nucleolar phosphoprotein B23, numatrin) pseudogene, and a BCR (breakpoint cluster region) pseudogene |
| PPAT | — | — | Homo sapiens similar to phosphoribosyl pyrophosphate amiodotransferase (LOC151641), mRNA |
| ARL8 | Chr10 | — | Sapiens mRNA; cDNA DKFZp43401317 (from clone DKFZp43401317)/ADP-ribosylation factor-like 8 |
| C20orf168 (HNRPA1) | Chr 20 | — | Human DNA sequence from clone RP3-447F3 on chromosome 20 and contains TNNC2 (troponin C2, fast) gene |
Gene name . | Chromosome location . | Predicted size . | Comments . |
|---|---|---|---|
| ZNF43 | 19p13.11 | 803 AA | Zinc finger proteins that share high sequence homology and likely function as transcriptional repressors; also known to be expressed in early development |
| ZNF257 | 19q13ÊÊ | 535 AA | |
| ZNF117 | 7q11.21 | 418 AA | |
| MGC27165 | Chr 14 | 5155 bases | Related to the fragilis group of proteins known to be germ cell–specific and critical for their maintenance |
| GSH1 | Chr 13 | 792 bases | GSH1 homeodomain protein containing a HOX domain |
| 264 AA | |||
| FLJ12581 (Nanog) | 12p13.31 | 6660 bases | ES cell–specific transcription factor critical for pluripotency |
| KIAA1573 | 1p13.3 | 3559 bases | Low homology to calcium channel, voltage-dependent, alpha 2/delta subunit 2 |
| Ribosomal 40A Laminin receptor | Chr 6 | — | Human DNA sequence from clone RP3-334F4 on chromosome 6 contains ESTs, STSs, and GSS: contains a LAMR1 (laminin receptor 1, ribosomal protein SA) pseudogene and an RPL10 (ribosomal protein L 10) pseudogene |
| C15orf15 | 15q21 | 15 719 bases | Chromosome 15 open reading frame 15 |
| C20orf129 | 20q11.22-q12 | 26 744 bases | Chromosome 20 open reading frame 129 |
| C20orf1 | 20q11.2 | 62 472 bases | Chromosome 20 open reading frame 1 |
| Numatrin | Chr 13 | — | Human DNA sequence from clone RP11-248N6 on chromosome 13 contains ESTs, STSs, and GSSs; contains 2 olfactory receptor pseudogenes, an NPM1 (nucleophosmin, nucleolar phosphoprotein B23, numatrin) pseudogene, and a BCR (breakpoint cluster region) pseudogene |
| PPAT | — | — | Homo sapiens similar to phosphoribosyl pyrophosphate amiodotransferase (LOC151641), mRNA |
| ARL8 | Chr10 | — | Sapiens mRNA; cDNA DKFZp43401317 (from clone DKFZp43401317)/ADP-ribosylation factor-like 8 |
| C20orf168 (HNRPA1) | Chr 20 | — | Human DNA sequence from clone RP3-447F3 on chromosome 20 and contains TNNC2 (troponin C2, fast) gene |
Nanog and 15 other novel genes overexpressed in 6 huES cell lines were analyzed by using bioinformatic tools available at multiple databases, including Swiss protein, Gencard, and Locus Link from the National Center for Biotechnology Information (NCBI), the National Cancer Institute, and the Center for Information Technology, National Institutes of Health. Chromosomal localization, predicted size, and possible function are shown. AA indicates amino acids; and—, not found.