EST enumeration and RT-PCR analysis of genes reported to be specific to mouse ES cells
Mouse gene . | Human homolog . | Array A/P/E/NE . | EST E/NF . | RT-PCR . | Comments . |
|---|---|---|---|---|---|
| POU5F1*† | Z11898 | E | E | + | ES cell marker |
| TDGF1/Cripto*† | X13293 | E | E | + | ES cell marker |
| Nanog*† | Hs.326290 | E | E | + | ES cell marker |
| GDF-3*† | Hs.86232 | E | NF | + | Expression confirmed by focused array |
| Utf1* | AB011076 | P/E | NF | + | Undifferentiated embryonic cell transcription factor 1 |
| Brachyury | Hs.143507 | P/NE | NF | + | Early mesodermal marker |
| DNMT3L | Hs.157237 | P/NE | E | + | Low but detected |
| HNRNPG-T | Hs.121605 | P/NE | NF | + | Testis-specific ring finger protein |
| Rex/Zfp42* | gi: 1082557 | P/NE | NF | + | ES cell marker |
| Dax-1/Nrob 1 | Hs.268490 | P/NE | NF | + | Nuclear receptor of unknown function |
| Keratin-17 | Hs.2785/Z19574 | P/NE | E | − | KRT-8/18 present but not KRT-17 |
| Tudor protein | AK010714.1 | A | NF | + | Maternal protein high in mouse ES cells |
| ESG1/DPPA5 | (cloned) | A | NF | + | Embryonic stem cell–specific gene |
| Fbx15 | Hs.124087 | P/NE | NF | − | F-box containing protein expressed in embryoid bodies regulated by Oct 3/4 |
| Zfp296 (Znf342) | Hs.192237 | A | NF | ND | Other zinc finger proteins are present in huES cells |
| Zfp226 | Sp: QGNYT6 | A | E | ND | Tex-20 or spalt like; detected in differentiating cells |
| E-ras Mm.249624 | AB093575 | A | NF | ND | huES cells express Ha-ras but no Eras detected |
| TCL1 | NP068801.1 | NP | NP | ND | Low homology to tcl1 (56%) |
| PRB1 (Mm.157658) | No human homolog | NP | NP | ND | Low homology to proline-rich protein PRB1 |
| Mm.45676 (EST) | No human homolog | NP | NP | ND | EST with low homology to Drosophila pipsqueak |
| E-Hox Mm.021300 | No human homolog | NP | NP | ND | No ortholog identified in human (2 paralogues on X chromosome) |
Mouse gene . | Human homolog . | Array A/P/E/NE . | EST E/NF . | RT-PCR . | Comments . |
|---|---|---|---|---|---|
| POU5F1*† | Z11898 | E | E | + | ES cell marker |
| TDGF1/Cripto*† | X13293 | E | E | + | ES cell marker |
| Nanog*† | Hs.326290 | E | E | + | ES cell marker |
| GDF-3*† | Hs.86232 | E | NF | + | Expression confirmed by focused array |
| Utf1* | AB011076 | P/E | NF | + | Undifferentiated embryonic cell transcription factor 1 |
| Brachyury | Hs.143507 | P/NE | NF | + | Early mesodermal marker |
| DNMT3L | Hs.157237 | P/NE | E | + | Low but detected |
| HNRNPG-T | Hs.121605 | P/NE | NF | + | Testis-specific ring finger protein |
| Rex/Zfp42* | gi: 1082557 | P/NE | NF | + | ES cell marker |
| Dax-1/Nrob 1 | Hs.268490 | P/NE | NF | + | Nuclear receptor of unknown function |
| Keratin-17 | Hs.2785/Z19574 | P/NE | E | − | KRT-8/18 present but not KRT-17 |
| Tudor protein | AK010714.1 | A | NF | + | Maternal protein high in mouse ES cells |
| ESG1/DPPA5 | (cloned) | A | NF | + | Embryonic stem cell–specific gene |
| Fbx15 | Hs.124087 | P/NE | NF | − | F-box containing protein expressed in embryoid bodies regulated by Oct 3/4 |
| Zfp296 (Znf342) | Hs.192237 | A | NF | ND | Other zinc finger proteins are present in huES cells |
| Zfp226 | Sp: QGNYT6 | A | E | ND | Tex-20 or spalt like; detected in differentiating cells |
| E-ras Mm.249624 | AB093575 | A | NF | ND | huES cells express Ha-ras but no Eras detected |
| TCL1 | NP068801.1 | NP | NP | ND | Low homology to tcl1 (56%) |
| PRB1 (Mm.157658) | No human homolog | NP | NP | ND | Low homology to proline-rich protein PRB1 |
| Mm.45676 (EST) | No human homolog | NP | NP | ND | EST with low homology to Drosophila pipsqueak |
| E-Hox Mm.021300 | No human homolog | NP | NP | ND | No ortholog identified in human (2 paralogues on X chromosome) |
Expression of 21 human homologs of murine ES cell–specific genes was analyzed by microarray, EST enumeration, and RT-PCR analysis.
A indicates absent; E, expressed; P, present on chip; NE, not expressed; NF, not found; ND, not done; NP, not performed (array and EST analysis could not be performed as no human homolog is available); +, positive; and −, negative.
Known ES cell markers in undifferentiated human ES cells that could be confirmed.
Known ES cell markers expressed at ≥ 3-fold higher than huURNA.